Experimental study of moiré method in laser scanning confocal microscopy

TLDR: In this article, a novel class of moire patterns, i.e., LSCM moire pattern, is presented and investigated by experiment by integrating moire method with laser scanning confocal microscopy (LSCM).

Abstract: By integrating moire method with laser scanning confocal microscopy (LSCM), a novel class of moire patterns, i.e., LSCM moire patterns, is presented and investigated by experiment in this article. The moire patterns are formed by the interference of a 1200line∕mm cross-line holographic grating and the scanning lines of LSCM. The principles and conditions of forming LSCM moire are described in detail. The measured parameters of moire fringes from experimental images agree well with theoretical analysis. The experimental results verify the feasibility and reality of the proposed moire method to be another effective and high accuracy technique for measuring and observing in-plane microdeformation at micrometer scale.