Journal ArticleDOI
Expression and isoforms of gp43 in different strains of Paracoccidioides brasiliensis
TLDR
The results strongly suggest an epitopic conservation among all isoforms analysed, since all of them were recognized by anti-gp43 monoclonal antibodies.Abstract:
Individual exoantigens from 13 Paracoccidioides brasiliensis isolates were obtained and tested against a panel of 50 sera from patients with paracoccidioidomycosis by immunodiffusion test. The index of positivity varied from 20 to 100% according to the isolate. When these exoantigens were analysed by SDS-PAGE, eight presented high amounts of the glycoprotein gp43, two presented small amounts and in three there was no detectable gp43. The eight isolates presenting high amounts of gp43 were submitted to isoelectric focusing, blotted to nitrocellulose membranes, and revealed by monoclonal and polyclonal anti-gp43 antibodies. Four gp43 isoform profiles were obtained: profile A presenting pIs of 6·0, 6·2, 6·6 and 7·0, profile B presenting pIs of 6·4, 6·8 and 7·2, profile C presenting pI > 8·5 and profile D, presenting pIs of 5·8, 6·2 and 6·6. In each pattern observed, a major band with a distinct pI was detected. Despite this variation, our results strongly suggest an epitopic conservation among all isoforms a...read more
Citations
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Journal ArticleDOI
Paracoccidioides brasiliensis and paracoccidioidomycosis: molecular approaches to morphogenesis, diagnosis, epidemiology, taxonomy and genetics.
TL;DR: The recognition of P. brasiliensis as an anamorph in the phylum Ascomycota, order Onygenales, family OnYgenaceae, has been accomplished by means of molecular tools, which has revised the taxonomic position of this fungus.
Journal ArticleDOI
An overview of the immunopathology of human paracoccidioidomycosis
TL;DR: The previous notion of the immune system as having two nearly independent arms, the innate and adaptive immunities, leaving a large gap between them, is now being overcome and immunologists are trying to dissect the connections between these two arms.
Journal ArticleDOI
Paracoccidioidomycosis: Current Perspectives from Brazil.
Rinaldo Poncio Mendes,Ricardo de Souza Cavalcante,Silvio Alencar Marques,Mariângela Esther Alencar Marques,James Venturini,Tatiane Fernanda Sylvestre,Anamaria Mello Miranda Paniago,Ana Carla Pereira,Julhiany de Fátima da Silva,Alexandre Todorovic Fabro,Sandra de Moraes Gimenes Bosco,Eduardo Bagagli,Rosane Christine Hahn,Adriele Dandara Levorato +13 more
TL;DR: Itraconazole and the trimethoprim-sulfamethoxazole combination was compared regarding efficacy, effectiveness and safety, demonstrating that azole should be the first choice in the treatment of paracoccidioidomycosis.
Journal ArticleDOI
Polymorphism in the gene coding for the immunodominant antigen gp43 from the pathogenic fungus Paracoccidioides brasiliensis.
TL;DR: The nucleotides encoding the inner core of the murine T-cell-protective epitope of gp43 were conserved, offering hope for the development of a universal vaccine.
Journal ArticleDOI
Isolation of a Paracoccidioides brasiliensis strain from the soil of a coffee plantation in Ibiá, State of Minas Gerais, Brazil
Mario León Silva-Vergara,Roberto Martinez,A. Chadu,M. Madeira,G. Freitas-Silva,C.M. Leite Maffei +5 more
TL;DR: The isolation of P. brasiliensis from the soil at a coffee plantation suggests that this is one of its habitats and supports the hypothesis of acquisition of paracoccidioidomycosis during agricultural activity in these areas.
References
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Journal ArticleDOI
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