Journal ArticleDOI
Extracellular and intracellular polygllacturonic acid trans-eliminases of Erwinia carotovora.
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TLDR
From the cells of the same organism, an intracellular enzyme wag isolated which has properties similar to those of the extra-cellular polygalacturonic acid trans-eliminase was purified from the culture fluid of Erwinia carolovora.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1968-02-01. It has received 177 citations till now. The article focuses on the topics: Pectinesterase.read more
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Book ChapterDOI
Pectin, pectinase and protopectinase: production, properties, and applications
TL;DR: Research has been initiated with the aim of developing an improved alternative microbial pectin production process by means of a specific enzyme, called “protopectinase,” which solubilizes protopectin forming highly polymerized soluble pECTin.
Journal ArticleDOI
Host-Pathogen Interactions : XXII. A Galacturonic Acid Oligosaccharide from Plant Cell Walls Elicits Phytoalexins.
TL;DR: The results suggest that dodeca-alpha-1,4-d-galacturonid is the active elicitor, but the possibility remains that the active component could be a slightly modified oligogalacturonide present, but not detected, in the purified fractions.
Journal ArticleDOI
Changes in activities of cell wall hydrolases during ethylene-induced ripening in banana: effect of 1-MCP, ABA, and IAA
TL;DR: In this paper, the effects of treatments with 1-methylcyclopropene (1-MCP), abscisic acid (ABA) and indole acetic acid (IAA) on the activities of various cell wall hydrolases were measured in order to help elucidate their roles during banana ripening.
Book ChapterDOI
Assay methods for pectic enzymes
TL;DR: Three assay procedures are described that permit quantitative assay of hydrolytic and β-eliminative pectic enzymes, respectively, and can guide purification strategies, particularly when used in conjunction with titration curves.
Journal ArticleDOI
Host-Pathogen Interactions XXV. Endopolygalacturonic Acid Lyase from Erwinia carotovora Elicits Phytoalexin Accumulation by Releasing Plant Cell Wall Fragments
TL;DR: It is demonstrated that endopolygalacturonic acid lyase elicits phytoalexin accumulation by releasing fragments from pectic polysaccharides in plant cell walls through heat-stable elicitor-active material solubilized from soybean cell walls.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification.
Journal ArticleDOI
Über die Bildung von ungesättigten Abbauprodukten durch ein pektinabbauendes Enzym
P. Albersheim,H. Neukom,H. Deuel +2 more
TL;DR: In a commercial pectinase preparation, an enzyme was found which attacked only the methyl ester of pectic acid as mentioned in this paper, which indicated the presence of Δ-4,5 unsaturated galacturonic acid groups.
Journal ArticleDOI
Studies relating to the purification and properties of pectin transeliminase.
Peter Albersheim,Ursula Killias +1 more
TL;DR: A convenient procedure for the purification of fungal pectin transeliminase (PTE) is described, taking advantage of the enzyme's low isoelectric point (pH 3.5), and a high degree of purification is obtained by column chromatography on DEAE cellulose.
Journal ArticleDOI
Eliminative split of pectic substances by phytopathogenic soft-rot bacteria.
Mortimer P. Starr,Francis Moran +1 more
TL;DR: Culture filtrates of Erwinia carotovora and Bacillus polymyxa split pectic substances to yield a product which absorbs at 230 m�, and which reacts with thiobarbituric acid to form a substance that absorbs at 547 m�.