Journal ArticleDOI
Fabrication of symmetric sub-5 nm nanopores using focused ion and electron beams
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TLDR
In this article, a focused ion beam (FIB) system was used to produce highly symmetrical nanopores with diameters smaller than 5nm and can be used to create arrays of multiple nanopores simultaneously.Abstract:
Nanopores fabricated in solid-state membranes have previously been used for the rapid electrical detection and characterization of single biopolymer molecules. Various methods for producing solid-state nanopores have been reported, but fabricating nanopores of desired sizes controllably is still challenging. Here we report a fabrication technique which uses a focused ion beam (FIB) system to engineer nanopores precisely. This technique provides visual feedback over the formation process. The present method can produce highly symmetrical nanopores with diameters smaller than ~5?nm and can be used to create an array of multiple nanopores simultaneously. In addition, nanopores produced using the focused ion beam sculpting technique can be tailored down to less than 1?nm in diameter using high-energy electron radiation.read more
Citations
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Journal ArticleDOI
Rapid Fabrication of Uniformly Sized Nanopores and Nanopore Arrays for Parallel DNA Analysis
TL;DR: In this article, a 3.5 nm pore a-hemolysin (a-HL) was used to detect double-stranded DNA molecules through 4 nm diameter nanopores by monitoring their translocation under an applied bias.
Journal ArticleDOI
Bioinspired smart asymmetric nanochannel membranes
TL;DR: A systematic overview of the development of the state-of-the-art BSANM system, covering most of the related literature reported since 2010, is presented to build up a broad and deep knowledge base that can provide a solid information source for the scientific community.
Journal ArticleDOI
Recapturing and trapping single molecules with a solid-state nanopore.
TL;DR: Repeated translocation of the same molecule improves measurement accuracy, offers a way to probe the chemical transformations and internal dynamics of macromolecules on sub-millisecond time and sub-micrometre length scales, and demonstrates the ability to trap, study and manipulate individual macromolescules in solution.
Journal ArticleDOI
Solid-state nanopore technologies for nanopore-based DNA analysis.
TL;DR: This review summarizes each solid-state nanopore fabrication technique reported to date, and compares their advantages and disadvantages, followed by a discussion of approaches used to measure pore size, geometry and surface properties.
Journal ArticleDOI
Rapid and precise scanning helium ion microscope milling of solid-state nanopores for biomolecule detection
J. Yang,David C. Ferranti,Lewis Stern,Colin A. Sanford,Jason Huang,Zheng Ren,Lu Chang Qin,Adam R. Hall +7 more
TL;DR: The fabrication process offers the advantage of high sample throughput along with fine control over nanopore dimensions, producing single pores with diameters below 4 nm, and the utility of the nanopores for biomolecular analysis is demonstrated by measuring the passage of double-strand DNA.
References
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Journal ArticleDOI
Characterization of individual polynucleotide molecules using a membrane channel
TL;DR: It is shown that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane, which could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.
Journal ArticleDOI
Ion-beam sculpting at nanometre length scales
Jiali Li,Derek Stein,Ciaran J. McMullan,Daniel Branton,Michael J. Aziz,Jene Andrew Golovchenko +5 more
TL;DR: It is shown that ion-beam sculpting can be used to fashion an analogous solid-state device: a robust electronic detector consisting of a single nanopore in a Si3N4 membrane, capable of registering single DNA molecules in aqueous solution.
Journal ArticleDOI
Fabrication of solid-state nanopores with single-nanometre precision.
Arnoldus Jan Storm,Jianghua Chen,Xinsheng Sean Ling,Xinsheng Sean Ling,Henny W. Zandbergen,Cees Dekker +5 more
TL;DR: A new technique for fabricating silicon oxide nanopores with single-nanometre precision and direct visual feedback, using state-of-the-art silicon technology and transmission electron microscopy is reported.
Journal ArticleDOI
Rapid nanopore discrimination between single polynucleotide molecules.
TL;DR: Because nanopores can rapidly discriminate and characterize unlabeled DNA molecules at low copy number, refinements of the experimental approach demonstrated here could eventually provide a low-cost high-throughput method of analyzing DNA polynucleotides.
Journal ArticleDOI
DNA molecules and configurations in a solid-state nanopore microscope.
TL;DR: A solid-state nanopore microscope capable of observing individual molecules of double-stranded DNA and their folding behaviour is demonstrated and extensions of the nanopore telescope concept to alternative probing mechanisms and applications are discussed, including the study of molecular structure and sequencing.