Journal ArticleDOI
Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria
Yuki Ichinose,Fumiko Taguchi,Masanobu Yamamoto,Mayumi Ohnishi-Kameyama,Tatsuo Atsumi,Tatsuo Atsumi,Masako Iwaki,Hiromi Manabe,Mio Kumagai,Quan Thanh Nguyen,Chi Linh Nguyen,Yoshishige Inagaki,Hiroshi Ono,Kazuhiro Chiku,Tadashi Ishii,Mitsuru Yoshida +15 more
TLDR
The results suggest that flagellin glycosylation is ubiquitous in most phytopathogenic bacteria and that flagescence is required for virulence in Xcc.Abstract:
Glycosylation of flagellin is known to be involved in filament stabilization, motility, and virulence in Pseudomonas
syringae. Here we investigated flagellin glycosylation in other phytopathogenic bacteria. Analyses of deduced amino acid sequences, glycostaining, and molecular masses of purified flagellins revealed that flagellins from all phytopathogenic bacteria investigated were glycosylated. Furthermore, the flagellin in a glycosylation-defective mutant of Xanthomonas campestris pv. campestris (Xcc) had a reduced molecular mass, and motility and virulence of the mutant toward host leaves decreased. These results suggest that flagellin glycosylation is ubiquitous in most phytopathogenic bacteria and that flagellin glycosylation is required for virulence in Xcc.read more
Citations
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Journal ArticleDOI
Bacterial flagella: twist and stick, or dodge across the kingdoms.
TL;DR: A wider appreciation is provided of the functions of bacterial flagella in the context of both plant and animal reservoirs: variation in their recognition impacts the course and outcome of infections in hosts from both kingdoms.
Journal ArticleDOI
Perception of pathogenic or beneficial bacteria and their evasion of host immunity: pattern recognition receptors in the frontline
Lucie Trdá,Lucie Trdá,Freddy Boutrot,Justine Claverie,Daphnée Brulé,Stéphan Dorey,Benoît Poinssot +6 more
TL;DR: This review overviews the RLKs and RLPs that assure early recognition and control of pathogenic or beneficial bacteria, and highlights the crucial function of PRRs during plant-microbe interactions, with a special emphasis on the receptors of the bacterial flagellin and peptidoglycan.
Journal ArticleDOI
Current Understandings of Plant Nonhost Resistance
Hyun-Ah Lee,Hye-Young Lee,Eunyoung Seo,Joohyun Lee,Saet-Byul Kim,Soohyun Oh,E. J. Choi,Eunhye Choi,So Eui Lee,Doil Choi +9 more
TL;DR: Overall, the current understanding of nonhost resistance mechanisms is not much deviated from the knowledge on host resistance, except for a few specific cases, and further insights on the roles of the pattern recognition receptor gene family, multiple interactions between effectors from nonadapted pathogen and plant factors, and plant secondary metabolites in host range determination are sought.
Journal ArticleDOI
Pathogenicity and virulence factors of Pseudomonas syringae
TL;DR: This work has attempted to characterize pathogenicity, the ability of a pathogen to cause disease, using the phytopathogenic bacterium Pseudomonas syringae as a representative pathogen.
Journal ArticleDOI
Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014
TL;DR: This review is the eighth update of the original article published in 1999 on the application of Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) mass spectromaetry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2014.
References
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Journal ArticleDOI
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum
TL;DR: Small mobilizable vectors based on the Escherichia coli plasmids pK18 and pK19 can be transferred by RP4-mediated conjugation into a wide range of Gram- and Gram+ bacteria, and should facilitate gene disruption and allelic exchange by homologous recombination.
Journal ArticleDOI
Preparation of Genomic DNA from Bacteria
TL;DR: This procedure is effective in producing digestible chromosomal DNA from a variety of gram-negative bacteria, all of which normally produce large amounts of polysaccharides.
Journal ArticleDOI
Comparison of the genomes of two Xanthomonas pathogens with differing host specificities
A.C.R. da Silva,Jesus Aparecido Ferro,Fernando C. Reinach,Chuck S. Farah,Luiz Roberto Furlan,Ronaldo Bento Quaggio,Claudia Barros Monteiro-Vitorello,M. A. Van Sluys,Nalvo F. Almeida,Lucia Maria Carareto Alves,A. M. do Amaral,Maria Célia Bertolini,Luis Eduardo Aranha Camargo,Giovana Camarotte,Fabiana de Souza Cannavan,Cardozo Jc,Felipe S. Chambergo,L. P. Ciapina,Regina Maria Barretto Cicarelli,Luiz Lehmann Coutinho,Jeny R. Cursino-Santos,Hamza El-Dorry,J. B. Faria,Ari J. S. Ferreira,Rita de Cássia Café Ferreira,Maria Inês Tiraboschi Ferro,Eduardo Fernandes Formighieri,Marília Caixeta Franco,Christian C. Greggio,Arthur Gruber,Angela M. Katsuyama,Luciano Takeshi Kishi,Rui P. Leite,Eliana Gertrudes de Macedo Lemos,Manoel Victor Franco Lemos,E. C. Locali,Marcos Antonio Machado,Alda Maria Backx Noronha Madeira,Nilce Maria Martinez-Rossi,E. C. Martins,João Meidanis,Carlos Frederico Martins Menck,Cristina Yumi Miyaki,D. H. Moon,Leandro Marcio Moreira,M. T. M. Novo,Vagner K. Okura,Mariana Cabral de Oliveira,V. R. Oliveira,H. A. Pereira,Antonio Rossi,Janete Apparecida Desidério Sena,Cícero Lopes da Silva,R. F. B. de Souza,L. A. F. Spinola,Marco Aurélio Takita,Rodrigo Esaki Tamura,E. C. Teixeira,R. I. D. Tezza,M. Trindade dos Santos,Daniela Truffi,Siu Mui Tsai,Frank F. White,Frank F. White,João C. Setubal,João Paulo Kitajima +65 more
TL;DR: The genus Xanthomonas is a diverse and economically important group of bacterial phytopathogens, belonging to the γ-subdivision of the Proteobacteria, and several groups of strain-specific genes are identified and proposed mechanisms that may explain the differing host specificities and pathogenic processes are proposed.
Book ChapterDOI
Preparation of Genomic DNA from Bacteria
TL;DR: This chapter describes two procedures for the isolation of chromosomal DNA from E. coli that can be used for most gram-negative and gram-positive bacteria or modified to isolate DNA from organisms other than bacteria.