Journal ArticleDOI
Fluorescence of the Purine and Pyrimidine Bases of the Nucleic Acids in Neutral Aqueous Solution at 300 degrees K
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TLDR
Fluorescence of adenine, guanine, cytosine, and uracil at room temperature in neutral aqueous solution has been detected by means of a digital signal accumulation technique as discussed by the authors.Abstract:
Fluorescence of adenine, guanine, cytosine, and uracil at room temperature in neutral aqueous solution has been detected by means of a digital signal accumulation technique. Corrected emission and excitation spectra are presented and compared with low-temperature data. The quantum yields are, respectively, 2.6 x 10(-4), 3.0 x 10(-4), 0.8 x 10(-4), and 0.5 x 10(-4) when the bases are excited at their low-energy absorption maxima.read more
Citations
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Journal ArticleDOI
Ultrafast Excited-State Dynamics in Nucleic Acids
TL;DR: The nature and dynamics of the singlet excited electronic states created in nucleic acids and their constituents by UV light are reviewed, finding that these states are highly stable to photochemical decay, perhaps as a result of selection pressure during a long period of molecular evolution.
Journal ArticleDOI
Nucleobase-specific quenching of fluorescent dyes. 1. nucleobase one-electron redox potentials and their correlation with static and dynamic quenching efficiencies
TL;DR: In this paper, the feasibility of photoinduced electron transfer reaction for the nucleobase-specific quenching of fluorescent dyes is investigated by the calculation of the standard free energy changes with the Rehm−Weller equation.
Journal ArticleDOI
DNA Excited-State Dynamics: From Single Bases to the Double Helix
Chris T. Middleton,Kimberly de La Harpe,Charlene Su,Yu Kay Law,Carlos E. Crespo-Hernández,Bern Kohler +5 more
TL;DR: Recent discoveries and controversies concerning the nature and dynamics of excited states in DNA model systems in solution are reviewed.
PatentDOI
Radiative decay engineering
TL;DR: In this paper, compositions and methods for increasing the intrinsic fluorescence intensity of biomolecules and low quantum yield fluorophores are described. And methods for the identification of nucleic acids are also provided.
Journal ArticleDOI
Excited states dynamics of DNA and RNA bases: characterization of a stepwise deactivation pathway in the gas phase.
TL;DR: The results could be rationalized according to the effect of the highly localized nature of the excitation combined to the presence of efficient deactivation pathway along both pyrimidine ring and amino group out-of-plane vibrational modes, which play a key role in the vibronic coupling between the initially excited pipi* and the dark npi* states.
References
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Journal ArticleDOI
Luminescence of Pyrimidines, Purines, Nucleosides, and Nucleotides at 77°K. The Effect of Ionization and Tautomerization
TL;DR: From a study of methylated pyrimidines it was shown that the lactim forms fluoresced at 300°K and phosphoresces at neutral pH at 77°K, and support the commonly accepted lactam structures for the natural pyridines.
Journal ArticleDOI
Fluorescence characteristics of purines, pyrimidines, and their derivatives: measurement of guanine in nucleic acid hydrolyzates.
TL;DR: Forescence spectrometry has been applied to the purines and pyrimidines revealing that many of them do emit appreciable fluorescence, and a simple and specific procedure for the fluorometric assay of guanine in nucleic acid hydrolyzates is reported.
Journal ArticleDOI
Excited States of Nucleotides and Singlet Energy Transfer in Polynucleotides
TL;DR: From a discussion of the various cases of intermolecular energy transfer for singlets, and considering the broadband spectra of the nucleotides, it is shown that Forster's ``very weak case'' is applicable.