Journal ArticleDOI
Fluorescent monitoring of SDS gel electrophoresis.
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TLDR
Reaction of proteins with dansyl chloride in mildly alkaline SDS systems at high mole ratios of dANSyl chloride to protein yields dansyated proteins that can be run in SDS gel electrophoresis systems with no significant change in protein mobility.About:
This article is published in Analytical Biochemistry.The article was published on 1971-11-01. It has received 169 citations till now. The article focuses on the topics: Gel electrophoresis of proteins & Gel electrophoresis.read more
Citations
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Human plasma epidermal growth factor/beta-urogastrone is associated with blood platelets.
TL;DR: Since hEGF/UG appears to be liberated from platelets during coagulation, platelet-associated EGF/UG may be involved in normal vascular and tissue repair and in the pathogenesis of atherosclerotic lesions.
Journal ArticleDOI
Two disulfide-linked polypeptide chains constitute the active F protein of paramyxoviruses
TL;DR: The finding that with three different paramyxoviruses the biologically active virions possess F1 and F2 polypeptides suggests that this is a general feature of paramyxOViruses, and that the activation of infectivity, cell fusion, and hemolysis is due to a conformational change in the F protein resulting from proteolytic cleavage to form an active complex of two disulfide-linked polypePTide chains.
Journal ArticleDOI
A thousand points of light: the application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics.
TL;DR: This review discusses problems encountered in the analysis of proteins visualized with conventional stains and addresses advances in fluorescence protein detection, including immunoblotting, as well as the use of charge‐coupled device (CCD) camera‐based and laser‐scanner‐based image acquisition devices in proteomics.
Journal ArticleDOI
Synthesis and secretion of corticotropins, melanotropins, and endorphins by rat intermediate pituitary cells.
Richard E. Mains,Betty A. Eipper +1 more
TL;DR: With rat intermediate pituitary cells, pulse-chase experiments utilizing [35S]methionine demonstrated almost quantitative conversion of the 30K precursor into labeled proteins similar to beta-endorphin and alpha-melanocyte-stimulating hormone.
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Two partially homologous adjacent light-inducible maize chloroplast genes encoding polypeptides of the P700 chlorophyll a-protein complex of photosystem I.
L E Fish,Ulrich Kück,L Bogorad +2 more
TL;DR: The extensive homology between maize polypeptides A1 and A2 suggests that both may be components of CPI, although CPI has been generally considered to be comprised of two molecules of a single protein of only 66 to 70 kDa.
References
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Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels
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A method for immediate visualization of proteins in acrylamide gels and its use for preparation of antibodies to enzymes.
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A highly sensitive procedure for detection of histones in polyacrylamide gels.
Wayne Wray,Elton Stubblefield +1 more
TL;DR: A procedure for the assay of histones in polyacryamide gels has been developed which allows detection of 0.1 μg protein and has the capability that, if the gels are found to be “overloaded” by this sensitive procedure, they may be destained by conventional procedures in 7% acetic acid with no loss of resolution.
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