Journal ArticleDOI
Freezing-thawing induces alterations in histone H1-DNA binding and the breaking of protein-DNA disulfide bonds in boar sperm
TLDR
Results suggest that the boar-sperm nuclear structure is heterogeneous suggesting the existence of a zonated pattern, differing in their total DNA density and the compactness of the precise nucleoprotein structures present in each zone.About:
This article is published in Theriogenology.The article was published on 2011-11-01. It has received 79 citations till now. The article focuses on the topics: Histone H1 & Protamine.read more
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Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs.
TL;DR: The present work summarizes the principles of cryoinjury and the relevance of permeating and nonpermeating cryoprotective agents and speculates with new research horizons on the preservation of boar sperm, such as vitrification and freeze-drying.
Journal ArticleDOI
Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches.
Maryam Hezavehei,Mohsen Sharafi,Homa Mohseni Kouchesfahani,Ralf Henkel,Ashok Agarwal,Vahid Esmaeili,Abdolhossein Shahverdi +6 more
TL;DR: Current freezing techniques and novel strategies that have been developed for sperm protection against cryo-damage are discussed, as well as evaluating the probable effects of sperm freezing on offspring health.
Journal ArticleDOI
Cryotolerance of stallion spermatozoa is related to ROS production and mitochondrial membrane potential rather than to the integrity of sperm nucleus
TL;DR: It is concluded that good and poor freezability stallion ejaculates differ in their reactive oxygen species levels after cryopreservation, but not in the damage extent on sperm nucleus.
Journal ArticleDOI
Recent Advances in Boar Sperm Cryopreservation: State of the Art and Current Perspectives
TL;DR: Most of these progresses have allowed counteracting better boar sperm cryodamage and are thus considered as forward steps for this storage method.
Journal ArticleDOI
Artificial insemination with frozen‐thawed boar sperm
TL;DR: In sum, artificial insemination with frozen‐thawed boar sperm can provide reasonable fertility outcomes, if freezable ejaculates, specific additives, and appropriate Insemination techniques are used.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
TL;DR: The detection of murine leukemia virus antigens in complex cellular lysates was used to demonstrate the efficacy of this electrophoretic transfer technique.
Journal ArticleDOI
Generation of reactive oxygen species, lipid peroxidation, and human sperm function.
TL;DR: Results are consistent with a causative role for lipid peroxidation in the etiology of defective sperm function and also suggest a possible physiological role for the reactive oxygen species generated by human spermatozoa in mediating sperm-zona interaction.
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