Functionalizing Ferritin Nanoparticles for Vaccine Development.
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TLDR
In this paper, a review of the building blocks that enable the formulation of ferritin-based vaccines at an experimental stage, including design, production, and purification are presented.Abstract:
In the last decade, the interest in ferritin-based vaccines has been increasing due to their safety and immunogenicity. Candidates against a wide range of pathogens are now on Phase I clinical trials namely for influenza, Epstein-Barr, and SARS-CoV-2 viruses. Manufacturing challenges related to particle heterogeneity, improper folding of fused antigens, and antigen interference with intersubunit interactions still need to be overcome. In addition, protocols need to be standardized so that the production bioprocess becomes reproducible, allowing ferritin-based therapeutics to become readily available. In this review, the building blocks that enable the formulation of ferritin-based vaccines at an experimental stage, including design, production, and purification are presented. Novel bioengineering strategies of functionalizing ferritin nanoparticles based on modular assembly, allowing the challenges associated with genetic fusion to be circumvented, are discussed. Distinct up/down-stream approaches to produce ferritin-based vaccines and their impact on production yield and vaccine efficacy are compared. Finally, ferritin nanoparticles currently used in vaccine development and clinical trials are summarized.read more
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References
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Journal ArticleDOI
Electron density map of apoferritin at 2.8-A resolution.
TL;DR: The 6-Å resolution structure of apoferritin3 showed the presence of several rods of electron density, tentatively assigned as α helices, and channels passing through the shell, which could provide an access route for Fe atoms, and these features have been confirmed at 2.8-™ resolution and can now also provide a plausible subunit conformation and quaternary structure.
Journal ArticleDOI
Chimeric ferritin nanocages for multiple function loading and multimodal imaging.
Xin Lin,Jin Xie,Gang Niu,Fan Zhang,Haokao Gao,Min Yang,Qimeng Quan,Maria A. Aronova,Guofeng Zhang,Seulki Lee,Richard D. Leapman,Xiaoyuan Chen +11 more
TL;DR: A good tumor targeting profile was observed, which was attributable to both the enhanced permeability and retention (EPR) effect and biovector mediated targeting, which promises ferritin particles as a powerful nanoplatfom in the era of nanomedicine.
Journal ArticleDOI
The ferritin superfamily: Supramolecular templates for materials synthesis.
TL;DR: Material scientists have been inspired by this system and exploited a range of ferritin superfamily proteins as supramolecular templates to encapsulate nanoparticles and/or as well-defined building blocks for fabrication of higher order assembly.
Journal ArticleDOI
Innate immune recognition of glycans targets HIV nanoparticle immunogens to germinal centers
Talar Tokatlian,Benjamin J. Read,Christopher A. Jones,Daniel W. Kulp,Daniel W. Kulp,Daniel W. Kulp,Sergey Menis,Sergey Menis,Jason Y. Chang,Jon M. Steichen,Jon M. Steichen,Sudha Kumari,Joel D. Allen,Eric L. Dane,Alessia Liguori,Maya Sangesland,Daniel Lingwood,Max Crispin,William R. Schief,Darrell J. Irvine +19 more
TL;DR: These findings identify an innate immune–mediated recognition pathway promoting antibody responses to particulate antigens, with broad implications for humoral immunity and vaccine design.
Journal ArticleDOI
Manipulating signaling at will: chemically-inducible dimerization (CID) techniques resolve problems in cell biology
TL;DR: In this article, the authors focus on case studies where CID was able to provide insight into otherwise refractory problems, such as lipid second messengers and small GTPases, and discuss several recent technical advances that provide improved specificity in CID action.