Further Insights into the Roles of GTP and the C Terminus of the Hepatitis C Virus Polymerase in the Initiation of RNA Synthesis
Deborah Harrus,Neveen Ahmed-El-Sayed,Philip C. Simister,Steve Miller,Martine Triconnet,Curt H. Hagedorn,Kathleen Mahias,Félix A. Rey,Thérèse Astier-Gin,Stéphane Bressanelli +9 more
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A combined structural and functional analysis of genotype 1 HCV-NS5b of strains H77, for which no structure has been previously reported, and J4, finds that GTP specifically stimulates this transition irrespective of its incorporation in neosynthesized RNA.About:
This article is published in Journal of Biological Chemistry.The article was published on 2010-10-22 and is currently open access. It has received 74 citations till now. The article focuses on the topics: RNA-dependent RNA polymerase & NS5B.read more
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Journal ArticleDOI
The molecular and structural basis of advanced antiviral therapy for hepatitis C virus infection
TL;DR: Insight into the structures of these proteins and the mechanisms by which they contribute to the HCV replication cycle are reviewed, and how these insights have facilitated the development of new, directly acting antiviral compounds that have started to enter the clinic are discussed.
Journal ArticleDOI
Structural basis for RNA replication by the hepatitis C virus polymerase.
Todd Appleby,Jason K. Perry,Eisuke Murakami,Ona Barauskas,Joy Y. Feng,Aesop Cho,David A. Fox,Diana R. Wetmore,Mary E. McGrath,Adrian S. Ray,Michael J. Sofia,S. Swaminathan,Thomas E. Edwards +12 more
TL;DR: The details of HCV RNA replication are studied by determining crystal structures of stalled polymerase ternary complexes with enzymes, RNA templates, RNA primers, incoming nucleotides, and catalytic metal ions during both primed initiation and elongation of RNA synthesis.
Book ChapterDOI
Hepatitis C virus proteins: from structure to function
Darius Moradpour,François Penin +1 more
TL;DR: This review summarizes current knowledge of the structure and function of the HCV proteins and highlights recent advances in the field.
Journal ArticleDOI
Hepatitis C virus RNA replication and assembly: living on the fat of the land.
TL;DR: The molecular mechanisms of viral replicase function, cellular pathways employed during HCV replication factory biogenesis, and viral, as well as cellular, determinants of progeny virus production are reviewed.
Book ChapterDOI
Hepatitis C Virus RNA Replication
TL;DR: This chapter summarizes the current knowledge on the role of viral and host cell proteins as well as cis-acting replication elements involved in the biogenesis of the membranous web and in viral RNA synthesis.
References
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Features and development of Coot.
TL;DR: Coot is a molecular-graphics program designed to assist in the building of protein and other macromolecular models and the current state of development and available features are presented.
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PHENIX: a comprehensive Python-based system for macromolecular structure solution
Paul D. Adams,Paul D. Adams,Pavel V. Afonine,Gábor Bunkóczi,Vincent B. Chen,Ian W. Davis,Nathaniel Echols,Jeffrey J. Headd,Li-Wei Hung,Gary J. Kapral,Ralf W. Grosse-Kunstleve,Airlie J. McCoy,Nigel W. Moriarty,Robert D. Oeffner,Randy J. Read,David S. Richardson,Jane S. Richardson,Thomas C. Terwilliger,Peter H. Zwart +18 more
TL;DR: The PHENIX software for macromolecular structure determination is described and its uses and benefits are described.
Book ChapterDOI
Phenix - a comprehensive python-based system for macromolecular structure solution
Paul D. Adams,Paul D. Adams,Pavel V. Afonine,Gábor Bunkóczi,Vincent B. Chen,Ian W. Davis,Nathaniel Echols,Jeffrey J. Headd,Li-Wei Hung,Gary J. Kapral,Ralf W. Grosse-Kunstleve,Airlie J. McCoy,Nigel W. Moriarty,Robert D. Oeffner,Randy J. Read,David S. Richardson,Jane S. Richardson,Thomas C. Terwilliger,Peter H. Zwart +18 more
TL;DR: PHENIX has been developed to provide a comprehensive system for macromolecular crystallographic structure solution with an emphasis on the automation of all procedures.
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CRYSOL : a program to evaluate X-ray solution scattering of biological macromolecules from atomic coordinates
TL;DR: In this paper, a program for evaluating the solution scattering from macromolecules with known atomic structure is presented, which uses multipole expansion for fast calculation of the spherically averaged scattering pattern and takes into account the hydration shell.
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Molecular replacement with MOLREP
Alexei A. Vagin,Alexei Teplyakov +1 more
TL;DR: MOLREP is an automated program for molecular replacement that utilizes a number of original approaches to rotational and translational search and data preparation that includes weighting of the X-ray data and search models, multi-copy search, fitting the model into electron density, structural superposition of two models and rigid-body refinement.