Genetic diversity of clinical and environmental strains of Salmonella enterica serotype Weltevreden isolated in Malaysia.
Kwai Lin Thong,Yee-Ling Goh,Son Radu,S. Noorzaleha,Rohani Md Yasin,Y.T. Koh,V. K. E. Lim,Gulam Rusul,Savithri D. Puthucheary +8 more
Reads0
Chats0
TLDR
Antibiotic sensitivity tests showed that the clinical isolates of Salmonella serotype Weltevreden remained drug sensitive but that the vegetable isolates were resistant to at least two antibiotics.Abstract:
The incidence of food-borne salmonellosis due to Salmonella enterica serotype Weltevreden is reported to be on the increase in Malaysia. The pulsed-field gel electrophoresis (PFGE) subtyping method was used to assess the extent of genetic diversity and clonality of Salmonella serotype Weltevreden strains from humans and the environment. PFGE of XbaI-digested chromosomal DNA from 95 strains of Salmonella serotype Weltevreden gave 39 distinct profiles with a wide range of Dice coefficients (0.27 to 1.00), indicating that PFGE is very discriminative and that multiple clones of Salmonella serotype Weltevreden exist among clinical and environmental isolates. Strains of one dominant pulsotype (pulsotype X1/X2) appeared to be endemic in this region, as they were consistently recovered from humans with salmonellosis between 1996 and 2001 and from raw vegetables. In addition, the sharing of similar PFGE profiles among isolates from humans, vegetables, and beef provides indirect evidence of the possible transmission of salmonellosis from contaminated raw vegetables and meat to humans. Furthermore, the recurrence of PFGE profile X21 among isolates found in samples of vegetables from one wet market indicated the persistence of this clone. The environment in the wet markets may represent a major source of cross-contamination of vegetables with Salmonella serotype Weltevreden. Antibiotic sensitivity tests showed that the clinical isolates of Salmonella serotype Weltevreden remained drug sensitive but that the vegetable isolates were resistant to at least two antibiotics. To the best of our knowledge, this is the first study to compare clinical and environmental isolates of Salmonella serotype Weltevreden in Malaysia.read more
Citations
More filters
Journal ArticleDOI
Application of Whole-Genome Sequencing for Bacterial Strain Typing in Molecular Epidemiology
Stephen J. Salipante,Dhruba J. Sengupta,Lisa A. Cummings,Tyler A. Land,Daniel R. Hoogestraat,Brad T. Cookson +5 more
TL;DR: It is demonstrated that PFGE is prone to false-positive and false-negative results and suggests the need for a new gold standard approach for molecular epidemiological strain typing, and bacterial whole-genome sequencing (WGS) is explored as an alternative approach for Molecular strain typing.
Journal ArticleDOI
Salmonella serovars from humans and other sources in Thailand, 1993-2002.
Aroon Bangtrakulnonth,Srirat Pornreongwong,Chaiwat Pulsrikarn,Pathom Sawanpanyalert,Rene S. Hendriksen,Danilo Lo Fo Wong,Frank Møller Aarestrup +6 more
TL;DR: The most common serovar causing human salmonellosis in Thailand was Salmonella enterica Weltevreden, which seems to be related toSalmonella serovars in different food products and reservoirs.
Journal ArticleDOI
Antimicrobial susceptibility and occurrence of resistance genes among Salmonella enterica serovar Weltevreden from different countries
Frank Møller Aarestrup,Monton Lertworapreecha,Mary Evans,Aroon Bangtrakulnonth,Thongchai Chalermchaikit,Rene S. Hendriksen,Henrik Caspar Wegener +6 more
TL;DR: A low frequency of resistance was found among Salmonella Weltevreden isolated from humans and other reservoirs in South-East Asia and elsewhere and there was no major difference in the occurrence of resistance between source or geographical origin.
Journal ArticleDOI
Prevalence and characterization of Salmonella enterica serovar Weltevreden from imported seafood.
Elizabeth Ponce,Elizabeth Ponce,Ashraf A. Khan,Chorng-Ming Cheng,Christine Summage-West,Carl E. Cerniglia +5 more
TL;DR: The findings show genetic diversity and temporal persistence of S. enterica serovar Weltevreden in recently monitored seafood imports and a low frequency of antibiotic resistance.
Journal ArticleDOI
Antimicrobial Resistance in Nontyphoidal Salmonella.
TL;DR: In this chapter, recent Salmonella antibiotic susceptibility data from Europe and the US are compared and the state of known resistance genes that have been identified in the genus are summarized.
References
More filters
Journal ArticleDOI
Antibiotic susceptibility testing by a standardized single disk method.
TL;DR: Recommendations of the National Committee for Clinical Laboratory Standards continue to be based on this publication; the “Kirby-Bauer” method is, among the many disk methods used in other countries, still the one that has been researched most thoroughly and updated continuously.
Journal ArticleDOI
Occurrence and Diversity of Tetracycline Resistance Genes in Lagoons and Groundwater Underlying Two Swine Production Facilities
TL;DR: It is demonstrated that tet genes occur in the environment as a direct result of agriculture and suggested that groundwater may be a potential source of antibiotic resistance in the food chain.
Book
Microbiological Safety and Quality of Food
TL;DR: Principles And Application Of Food Preservation Techniques: Production of Microbiologically Safe And Stable Foods, and Principles and Application of Predictive Modeling of the Effects of Preservative Factors on Microorganisms.
Journal ArticleDOI
An outbreak of multidrug-resistant, quinolone-resistant Salmonella enterica serotype typhimurium DT104
Kåre Mølbak,Dorte Lau Baggesen,Frank Møller Aarestrup,Jens Munk Ebbesen,Jørgen Engberg,Kai Frydendahl,Peter Gerner-Smidt,Andreas Petersen,Henrik Caspar Wegener +8 more
TL;DR: The investigation of an outbreak of DT104 documented the spread of quinolone-resistant bacteria from food animals to humans; this spread was associated with infections that were difficult to treat.
Journal ArticleDOI
Molecular Epidemiology: Application of Contemporary Techniques to the Typing of Microorganisms
TL;DR: This research presents a novel and scalable approach to promotor-free vaccination of canine coronavirus that aims to provide real-time information about the immune defences of the immune system and how these defences are damaged during infection.