Genetically engineered broad-spectrum disease resistance in tomato
TLDR
It is reported that the overexpression of Prf leads to enhanced resistance to a number of normally virulent bacterial and viral pathogens and leads to increased sensitivity to fenthion.Abstract:
Resistance in tomato to the bacterial pathogen Pseudomonas syringae pathovar tomato requires Pto and Prf. Mutations that eliminate Prf show a loss of both Pto resistance and sensitivity to the organophosphate insecticide fenthion, suggesting that Prf controls both phenotypes. Herein, we report that the overexpression of Prf leads to enhanced resistance to a number of normally virulent bacterial and viral pathogens and leads to increased sensitivity to fenthion. These plants express levels of salicylic acid comparable to plants induced for systemic acquired resistance (SAR) and constitutively express pathogenesis related genes. These results suggest that the overexpression of Prf activates the Pto and Fen pathways in a pathogen-independent manner and leads to the activation of SAR. Transgene-induced SAR has implications for the generation of broad spectrum disease resistance in agricultural crop plants.read more
Citations
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Plant pathogens and integrated defence responses to infection.
TL;DR: The current knowledge of recognition-dependent disease resistance in plants is reviewed, and a few crucial concepts are included to compare and contrast plant innate immunity with that more commonly associated with animals.
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Arabidopsis RIN4 Is a Target of the Type III Virulence Effector AvrRpt2 and Modulates RPS2-Mediated Resistance
TL;DR: RIN4 is a point of convergence for the activity of at least three unrelated P. syringae type III effectors, and is suggested to be the target of the AvrRpt2 virulence function, and that perturbation of RIN4 activates RPS2.
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How do plants achieve immunity? Defence without specialized immune cells
Steven H. Spoel,Xinnian Dong +1 more
TL;DR: Recent developments point towards a multilayered plant innate immune system comprised of self-surveillance, systemic signalling and chromosomal changes that together establish effective immunity.
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Understanding the functions of plant disease resistance proteins.
TL;DR: Many disease resistance (R) proteins of plants detect the presence of disease-causing bacteria, viruses, or fungi by recognizing specific pathogen effector molecules that are produced during the infection process.
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RESISTANCE GENE COMPLEXES: Evolution and Utilization
TL;DR: More than 30 genes have been characterized from different plant species that provide resistance to a variety of different pathogen and pest species and the structures of most are consistent with a role in pathogen recognition and defense response signaling.
References
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Journal ArticleDOI
A Receptor Kinase-Like Protein Encoded by the Rice Disease Resistance Gene, Xa21
Wen-Yuan Song,Guo-Liang Wang,Lili Chen,Han Suk Kim,Liya Pi,Tom Holsten,John M. Gardner,Bei Wang,Wen Xue Zhai,Li Huang Zhu,Claude M. Fauquet,Pamela C. Ronald +11 more
TL;DR: The rice Xa21 gene, which confers resistance to Xanthomonas oryzae pv. race 6, was isolated by positional cloning and the sequence of the predicted protein, which carries both a leucine-rich repeat motif and a serine-threonine kinase-like domain, suggests a role in cell surface recognition of a pathogen ligand and subsequent activation of an intracellular defense response.
Journal ArticleDOI
Systemic Acquired Resistance.
John Ryals,Urs Neuenschwander,Michael G. Willits,Antonio Molina,Henry-York Steiner,Michelle D. Hunt +5 more
A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. - eScholarship
W Y Song,G L Wang,L L Chen,H S Kim,L Y Pi,T Holsten,J Gardner,B Wang,W X Zhai,L H Zhu,C Fauquet,P Ronald +11 more
TL;DR: The sequence of the predicted protein, which carries both a leucine-rich repeat motif and a serine-threonine kinase-like domain, suggests a role in cell surface recognition of a pathogen ligand and subsequent activation of an intracellular defense response.
Journal ArticleDOI
Resistance gene-dependent plant defense responses.
TL;DR: The essential prerequisites for pathogen recognition and the induction of localized defense responses are examined, showing which responses are required to abolish or retard pathogen growth and how.
Journal ArticleDOI
Requirement of Salicylic Acid for the Induction of Systemic Acquired Resistance
Thomas Gaffney,Leslie Friedrich,Bernard Vernooij,David Vincent Negrotto,Gordon Nye,Scott Uknes,Eric R. Ward,Helmut Kessmann,John Ryals +8 more
TL;DR: Salicylic acid is essential for the development of systemic acquired resistance in tobacco and was investigated in transgenic tobacco plants harboring a bacterial gene encoding salicylate hydroxylase.