Journal ArticleDOI
Genomic alterations associated with malignancy in head and neck cancer
Ulrike Bockmühl,Günter Wolf,Sven Schmidt,Anke Schwendel,V. Jahnke,Manfred Dietel,Iver Petersen +6 more
TLDR
Comparative genomic hybridization was performed on 50 primary head and neck squamous cell carcinomas to discover molecular genetic alterations underlying the progression of these tumors.Abstract:
Background
Comparative genomic hybridization (CGH) was performed on 50 primary head and neck squamous cell carcinomas (HNSCC) to discover molecular genetic alterations underlying the progression of these tumors.
Methods
In CGH, equal amounts of differently labeled tumor deoxyribonucleic acid (DNA) and normal reference DNA were hybridized simultaneously to normal metaphase chromosomes. They were visualized by different fluorochromes, and the signal intensities were quantitated separately as gray levels along the single chromosomes. The over- and underrepresented DNA segments were determined by computation of ratio images and average ratio profiles.
Results
Prevalent changes observed in more than 50% of the HNSCC included deletions of chromosomes 1p, 4, 5q, 6q, 8p, 9p, 11, 13q, 18q, and 21q and DNA overrepresentations of 11q13 as well as 3q, 8q, 16p, 17q, 19, 20q, and 22q. The calculation of ratio profiles of tumor subgroups revealed that well differentiated carcinomas (G1) were defined by the deletions of chromosomes 3p, 5q, and 9p together with the overrepresentation of 3q, suggesting the association with early tumor development. Accordingly, the undifferentiated tumors (G3) were characterized by additional deletions of chromosomes 4q, 8p, 11q, 13q, 18q, 21q, and overrepresentations of 1p, 11q13, 19, and 22q.
Conclusion
Our data indicate that the CGH patterns of chromosomal imbalances may help to define the malignant potential of head and neck squamous cell carcinomas. © 1998 John Wiley & Sons, Inc. Head Neck20: 145–151, 1998.read more
Citations
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Pathology and genetics of tumours of the lung , pleura, thymus and heart
TL;DR: This book will not become a unity of the way for you to get amazing benefits at all, but, it will serve something that will let you get the best time and moment to spend for reading the book.
Journal ArticleDOI
The DNA sequence of human chromosome 21
Masahira Hattori,Asao Fujiyama,Todd D. Taylor,Hidemi Watanabe,Tetsushi Yada,H.-S. Park,Atsushi Toyoda,Kazuo Ishii,Yasushi Totoki,Dong-Kug Choi,Eiichi Soeda,Misao Ohki,T. Takagi,Yoshiyuki Sakaki,S. Taudien,K. Blechschmidt,A. Polley,U. Menzel,Jean-Maurice Delabar,K. Kumpf,R. Lehmann,David Patterson,Kathrin Reichwald,Andreas Rump,M. Schillhabel,A. Schudy,W. Zimmermann,André Rosenthal,Jun Kudoh,Kazunori Shibuya,Kazuhiko Kawasaki,Shuichi Asakawa,Ai Shintani,Takashi Sasaki,Kentaro Nagamine,Susumu Mitsuyama,Stylianos E. Antonarakis,Shinsei Minoshima,Nobuyoshi Shimizu,Gabriele Nordsiek,K. Hornischer,P. Brandt,M. Scharfe,O. Schön,A. Desario,J. Reichelt,G. Kauer,H. Blöcker,Juliane Ramser,Alfred Beck,Sven Klages,Steffen Hennig,L. Riesselmann,Emilie Dagand,Thomas Haaf,S. Wehrmeyer,K. Borzym,Katheleen Gardiner,Dean Nizetic,Fiona Francis,Hans Lehrach,Richard Reinhardt,Marie-Laure Yaspo +62 more
TL;DR: In this article, the authors reported the sequence and gene catalogue of the long arm of chromosome 21 and sequenced 33,546,361 base pairs (bp) of DNA with very high accuracy, the largest contig being 25,491,867 bp.
Journal ArticleDOI
Assessment of Genetic Changes in Hepatocellular Carcinoma by Comparative Genomic Hybridization Analysis: Relationship to Disease Stage, Tumor Size, and Cirrhosis
Nathalie Wong,Paul B.S. Lai,Siu-Wah Lee,Susanna Fan,Elizabeth Pang,Choong-Tsek Liew,Zhong Sheng,Joseph Wan-Yee Lau,Philip J. Johnson +8 more
TL;DR: It is suggested that 8q over-representation is likely associated with a growth advantage and proliferative stimulation that have encouraged malignant changes in the noncirrhotic human liver.
Journal ArticleDOI
Molecular pathogenesis of oral squamous carcinoma
TL;DR: Oral squamous carcinogenesis is a multistep process in which multiple genetic events occur that alter the normal functions of oncogenes and tumour suppressor genes, leading to a cell phenotype capable of increased cell proliferation, with loss of cell cohesion.
Journal ArticleDOI
Chromosomal alterations in squamous cell carcinomas of the head and neck: Window to the biology of disease
TL;DR: A brief description of the latest cytogenetic techniques and a description of chromosomal alterations in SCCHN, their molecular correlates, and clinical implications are presented.
References
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Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
Comparative Genomic Hybridization for Molecular Cytogenetic Analysis of Solid Tumors
Anne Kallioniemi,Olli Kallioniemi,Damir Sudar,Denis Rutovitz,Joe W. Gray,Fred Waldman,Daniel Pinkel +6 more
TL;DR: Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome, which identified 16 different regions of amplification, many in loci not previously known to be amplified.
Journal Article
Genetic progression model for head and neck cancer: Implications for field cancerization
Joseph A. Califano,Peter van der Riet,William H. Westra,Homaira Nawroz,Gary L. Clayman,Steven Piantadosi,Russell L. Corio,Daniel J. Lee,Benjamin Greenberg,Wayne M. Koch,David Sidransky +10 more
TL;DR: The local clinical phenomenon of field cancerization seems to involve the expansion and migration of clonally related preneoplastic cells within normal mucosal cells surrounding preinvasive and microinvasive lesions and thus appear to arise from a single progenitor clone.
Journal ArticleDOI
Estimates of the worldwide frequency of sixteen major cancers in 1980.
TL;DR: The numbers of new cancer cases in 16 common sites occurring in 1980 have been estimated and it is suggested that, with declining incidence rates for stomach cancer and the continuing rise for lung cancer, the latter would become the most common cancer in the world by the end of 1981.
Journal ArticleDOI
Detection and mapping of amplified DNA sequences in breast cancer by comparative genomic hybridization.
Anne Kallioniemi,Olli Kallioniemi,Jim Piper,Minna Tanner,Trond Stokke,Ling Chen,Helene S. Smith,Daniel Pinkel,Joe W. Gray,Frederic M. Waldman +9 more
TL;DR: Comparative genomic hybridization was applied to 5 breast cancer cell lines and 33 primary tumors to discover and map regions of the genome with increased DNA-sequence copy-number, indicating that these chromosomal regions may contain previously unknown genes whose increased expression contributes to breast cancer progression.