Genomic Background and Phylogeny of cfiA-Positive Bacteroides fragilis Strains Resistant to Meropenem-EDTA.
Sylvia Valdezate,Fernando Cobo,Sara Monzón,María J. Medina-Pascual,Ángel Zaballos,Isabel Cuesta,Silvia Pino-Rosa,Pilar Villalón +7 more
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TLDR
In this article, the genomes, taxonomy, and phylogenetic relationships with respect to other Bacteroides fragilis genomes of two resistant B. fragils strains (CNM20180471 and CNM20200206) were examined.Abstract:
Background: Bacteroides fragilis shows high antimicrobial resistance (AMR) rates and possesses numerous AMR mechanisms. Its carbapenem-resistant strains (metallo-β-lactamase cfiA-positive) appear as an emergent, evolving clade. Methods: This work examines the genomes, taxonomy, and phylogenetic relationships with respect to other B. fragilis genomes of two B. fragilis strains (CNM20180471 and CNM20200206) resistant to meropenem+EDTA and other antimicrobial agents. Results: Both strains possessed cfiA genes (cfiA14b and the new cfiA28), along with other AMR mechanisms. The presence of other efflux-pump genes, mexAB/mexJK/mexXY-oprM, acrEF/mdtEF-tolC, and especially cusR, which reduces the entry of carbapenem via the repression of porin OprD, may be related to meropenem–EDTA resistance. None of the detected insertion sequences were located upstream of cfiA. The genomes of these and other B. fragilis strains that clustered together in phylogenetic analyses did not meet the condition of >95% average nucleotide/amino acid identity, or >70% in silico genome-to-genome hybridization similarity, to be deemed members of the same species, although <1% difference in the genomic G+C content was seen with respect to the reference genome B. fragilis NCTC 9343T. Conclusions: Carbapenem-resistant strains may be considered a distinct clonal entity, and their surveillance is recommended given the ease with which they appear to acquire AMR.read more
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Time for Some Group Therapy: Update on Identification, Antimicrobial Resistance, Taxonomy, and Clinical Significance of the Bacteroides fragilis Group
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Antimicrobial Susceptibility and Clinical Findings of Anaerobic Bacteria
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Phenotypic and genotypic identification of carbapenem resistance in Bacteroides fragilis clinical strains
TL;DR: The Carba NP test results were positive for all the cfiA-positive B. fragilis isolates, including those susceptible to carbapenems based on their MIC values as discussed by the authors .
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Development and Clinical Application of a Multilocus Sequence Typing Scheme for Bacteroides fragilis Based on Whole-Genome Sequencing Data
TL;DR: In this article , a multilocus sequence typing (MLST) scheme was proposed for Bacteroides fragilis, one of the most abundant pathogenic bacteria in the human gut microbiota.
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Anaerobic Profile of Intra-abdominal Infections – A 23-Year Retrospective Study
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