Genomic characterization of multidrug-resistant Salmonella serovar Kentucky ST198 isolated in poultry flocks in Spain (2011–2017)
Clara Samper-Cativiela,Bernabé Diéguez-Roda,Filipa Trigo da Roza,María Ugarte-Ruiz,Ehud Elnekave,Seung-Ryeol Lim,M. Candelaria Vega Hernandez,David Abad,Soledad Collado,Jose Luis Saez,Cristina de Frutos,Montserrat Agüero,Miguel A. Moreno,Jose Antonio Escudero,Julio Alvarez +14 more
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The results indicate that the MDR S. Kentucky ST198 is present in all investigated poultry hosts in Spain, and that certain strains also carry additional plasmid-mediated ARGs, thus increasing its potential public health significance.Abstract:
Salmonella Kentucky is commonly found in poultry and rarely associated with human disease. However, a multidrug-resistant (MDR) S. Kentucky clone [sequence type (ST)198] has been increasingly reported globally in humans and animals. Our aim here was to assess if the recently reported increase of S. Kentucky in poultry in Spain was associated with the ST198 clone and to characterize this MDR clone and its distribution in Spain. Sixty-six isolates retrieved from turkey, laying hen and broiler in 2011–2017 were subjected to whole-genome sequencing to assess their sequence type, genetic relatedness, and presence of antimicrobial resistance genes (ARGs), plasmid replicons and virulence factors. Thirteen strains were further analysed using long-read sequencing technologies to characterize the genetic background associated with ARGs. All isolates belonged to the ST198 clone and were grouped in three clades associated with the presence of a specific point mutation in the gyrA gene, their geographical origin and isolation year. All strains carried between one and 16 ARGs whose presence correlated with the resistance phenotype to between two and eight antimicrobials. The ARGs were located in the Salmonella genomic island (SGI-1) and in some cases (bla SHV-12 , catA1, cmlA1, dfrA and multiple aminoglycoside-resistance genes) in IncHI2/IncI1 plasmids, some of which were consistently detected in different years/farms in certain regions, suggesting they could persist over time. Our results indicate that the MDR S. Kentucky ST198 is present in all investigated poultry hosts in Spain, and that certain strains also carry additional plasmid-mediated ARGs, thus increasing its potential public health significance.read more
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Epidemiological links and antimicrobial resistance of clinical Salmonella enterica ST198 isolates: a nationwide microbial population genomic study in Switzerland
Michael Biggel,Jule Anna Horlbog,Magdalena Nüesch-Inderbinen,Marie Anne Chattaway,Roger Stephan +4 more
TL;DR: The results emphasize the urgent need for implementing whole-genome sequencing as a routine tool for Salmonella surveillance and outbreak detection and to understand the genomic diversity and antimicrobial resistance gene content associated with S. Kentucky.
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Antibiotic Resistance Genes Occurrence in Conventional and Antibiotic-Free Poultry Farming, Italy
Muhammad Farooq,Camilla Smoglica,Fausto Ruffini,L. De Soldati,Fulvio Marsilio,Cristina Esmeralda Di Francesco +5 more
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Prevalence, antibiotic susceptibility and genomic analysis of Salmonella from retail meats in Shaanxi, China.
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Antimicrobial Susceptibility and Resistance Mechanisms in Mannheimia haemolytica Isolates from Sheep at Slaughter
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TL;DR: In this paper , the authors investigated the levels of antimicrobial resistance of M. haemolytica isolated from clinically healthy sheep at slaughter and the genetic bacterial resistance mechanisms in cattle and sheep.
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