Homogeneous Scoring of Single-Nucleotide Polymorphisms: Comparison of the 5′-Nuclease TaqMan® Assay and Molecular Beacon Probes
TLDR
Two homogeneous assays based on real-time fluorescence monitoring during PCR are relevant alternatives for large-scale genotyping of single-nucleotide polymorphisms (SNPs) using three SNPs in the human estrogen receptor gene as targets.Abstract:
Homogeneous assays based on real-time fluorescence monitoring during PCR are relevant alternatives for large-scale genotyping of single-nucleotide polymorphisms (SNPs). We compared the performance ...read more
Citations
More filters
Journal ArticleDOI
Accessing genetic variation: genotyping single nucleotide polymorphisms
TL;DR: The hope that single nucleotide polymorphisms will allow genes that underlie complex disease to be identified, together with progress in identifying large sets ofSNPs, are the driving forces behind intense efforts to establish the technology for large-scale analysis of SNPs.
Real-Time PCR
TL;DR: The real-time polymerase chain reaction (PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction, which combines the DNA amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect amplification products.
Journal ArticleDOI
Genotyping-by-sequencing (GBS), an ultimate marker-assisted selection (MAS) tool to accelerate plant breeding.
TL;DR: Genotyping-by-sequencing (GBS) has been developed and applied in sequencing multiplexed samples that combine molecular marker discovery and genotyping and has been successfully used in implementing genome-wide association study (GWAS), genomic diversity study, genetic linkage analysis, molecular markers discovery and genomic selection under a large scale of plant breeding programs.
Journal ArticleDOI
A new class of homogeneous nucleic acid probes based on specific displacement hybridization
TL;DR: A new class of probes for homogeneous nucleic acid detection based on the proposed displacement hybridization that demonstrates that multiple targets can be distinguished in the same solution, even if they differ from one another by as little as a single nucleotide.
Journal ArticleDOI
Diagnosis of Hepatitis A Virus Infection: a Molecular Approach
TL;DR: Current serologic tests provide the foundation for diagnosis of hepatitis A and hepatitis A virus (HAV) infection and new diagnostic methods have provided tools for the rapid detection of food-borne HAV transmission and identification of the potential source of the food contamination.
References
More filters
Journal ArticleDOI
Molecular Beacons: Probes that Fluoresce upon Hybridization
Sanjay Tyagi,Fred Russell Kramer +1 more
TL;DR: Novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions that undergo a spontaneous conforma-tional change when they hybridize to their targets are developed.
Journal ArticleDOI
Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.
TL;DR: The 5'----3' exonuclease activity of the thermostable enzyme Thermus aquaticus DNA polymerase may be employed in a polymerase chain reaction product detection system to generate a specific detectable signal concomitantly with amplification.
Journal ArticleDOI
Large-Scale Identification, Mapping, and Genotyping of Single-Nucleotide Polymorphisms in the Human Genome
David G. Wang,Jian-Bing Fan,Jian-Bing Fan,Chia-Jen Siao,Chia-Jen Siao,Anthony Berno,Anthony Berno,Peter M. Young,Peter M. Young,Ron Sapolsky,Ron Sapolsky,Ghassan Ghandour,Ghassan Ghandour,Nancy Perkins,Nancy Perkins,Ellen Winchester,Ellen Winchester,Jessica B. Spencer,Jessica B. Spencer,Leonid Kruglyak,Leonid Kruglyak,Lincoln Stein,Lincoln Stein,Linda Hsie,Linda Hsie,Thodoros Topaloglou,Thodoros Topaloglou,Earl Hubbell,Earl Hubbell,Elizabeth M. Robinson,Elizabeth M. Robinson,Michael P. Mittmann,Michael P. Mittmann,Macdonald S. Morris,Macdonald S. Morris,Naiping Shen,Naiping Shen,Dan Kilburn,Dan Kilburn,John D. Rioux,John D. Rioux,Chad Nusbaum,Chad Nusbaum,Steve Rozen,Steve Rozen,Thomas J. Hudson,Thomas J. Hudson,Robert J. Lipshutz,Robert J. Lipshutz,Mark S. Chee,Mark S. Chee,Eric S. Lander,Eric S. Lander +52 more
TL;DR: A large-scale survey for SNPs was examined by a combination of gel-based sequencing and high-density variation-detection DNA chips, and a genetic map was constructed showing the location of 2227 candidate SNPs.
Journal ArticleDOI
Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.
TL;DR: It is proposed that the larger signal in the 5' nuclease PCR assay is caused by increased likelihood of cleavage by Taq DNA polymerase when the probe is hybridized to a template strand during PCR.
Journal ArticleDOI
Multicolor molecular beacons for allele discrimination
TL;DR: Using several molecular beacons, each designed to recognize a different target and each labeled with a different fluorophore, it is demonstrated that multiple targets can be distinguished in the same solution, even if they differ from one another by as little as a single nucleotide.