Human gamma-trace, a basic microprotein: amino acid sequence and presence in the adenohypophysis
Anders Grubb,Helge Löfberg +1 more
TLDR
The tissue localization and amino acid sequence of gamma-trace indicated that this protein is connected with the peptidergic gastroenteropancreatic neuroendocrine system.Abstract:
The amino acid sequence of human gamma-trace, a basic microprotein without known function, was determined by automated Edman degradations of the carboxymethylated polypeptide chain and of fragments obtained by cyanogen bromide treatment and tryptic digestion after blocking of lysine residues. The single polypeptide chain contained 120 residues, and the calculated Mr was 13,260. A proline residue at position 3 was partly hydroxylated. The presence of gamma-trace in a significant proportion of the cells in the anterior lobe of simian and human pituitary glands was demonstrated by immunohistochemical procedures with a rabbit antiserum against human gamma-trace. The tissue localization and amino acid sequence of gamma-trace indicated that this protein is connected with the peptidergic gastroenteropancreatic neuroendocrine system.read more
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Cysteine cathepsins: From structure, function and regulation to new frontiers
TL;DR: The view of cysteine cathepsins as lysosomal proteases is changing as there is now clear evidence of their localization in other cellular compartments, and some of the remarkable advances that have taken place in the past decade are presented.
Journal ArticleDOI
The cystatins: protein inhibitors of cysteine proteinases.
Vito Turk,Wolfram Bode +1 more
TL;DR: Recently determined crystal structures of chicken cystatin and human stefin B established a new mechanism of interaction between cysteine proteinases and their inhibitors which is fundamentally different from the standard mechanism for serine proteinase inhibitors and their inhibitor.
Journal ArticleDOI
Serum cystatin C measured by automated immunoassay: A more sensitive marker of changes in GFR than serum creatinine
David J. Newman,Hansa Thakkar,Robert G. Edwards,Martin Wilkie,Thomas White,Anders Grubb,Christopher P. Price +6 more
TL;DR: Cystatin C measurement using PETIA technology can be automated on the same instruments used routinely for the measurement of creatinine and offers better analytical performance and probably improved clinical sensitivity as a screening test for early renal damage.
Journal ArticleDOI
Cystatin C as a marker of GFR--history, indications, and future research.
TL;DR: A multinational expert meeting was held in April 2002 in Marburg, Germany as discussed by the authors to summarize recent knowledge on the protein cystatin C (cys-C) and its use as a marker of the glomerular filtration rate (GFR).
Journal ArticleDOI
Serum cystatin C, determined by a rapid, automated particle-enhanced turbidimetric method, is a better marker than serum creatinine for glomerular filtration rate.
Jan Kyhse-Andersen,Camilla Schmidt,Gunnar Nordin,Britta Andersson,Peter Nilsson-Ehle,Veronica Lindström,Anders Grubb +6 more
TL;DR: A fully automated particle-enhanced turbidimetric assay for cystatin C in undiluted serum and EDTA-plasma that seems an attractive alternative to creatinine for estimation of GFR.
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