Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART
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TLDR
Sperm cryopreservation is an important component of fertility management and much of its successful application seems to affect the reproductive outcome of assisted reproduction technologies (ART): appropriate use of cryoprotectants before and sperm selection technologies after cryop Reservation seem to have the greatest impact on preventing DNA fragmentation, thus improving sperm cryosurvival rates.Abstract:
Cryopreservation of human spermatozoa—introduced in the 1960's—has been recognized as an efficient procedure for management of male fertility before therapy for malignant diseases, vasectomy or surgical infertility treatments, to store donor and partner spermatozoa before assisted reproduction treatments and to ensure the recovery of a small number of spermatozoa in severe male factor infertility. Despite the usefulness of it, cryopreservation may lead to deleterious changes of sperm structure and function: while the effects of cryopreservation on cells are well documented, to date there is no agreement in the literature on whether or not cryopreservation affects sperm chromatin integrity or on the use of a unique and functional protocol for the freezing-thawing procedure. Therefore, sperm cryopreservation is an important component of fertility management and much of its successful application seems to affect the reproductive outcome of assisted reproduction technologies (ART): appropriate use of cryoprotectants before and sperm selection technologies after cryopreservation seem to have the greatest impact on preventing DNA fragmentation, thus improving sperm cryosurvival rates.read more
Citations
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Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches.
Maryam Hezavehei,Mohsen Sharafi,Homa Mohseni Kouchesfahani,Ralf Henkel,Ashok Agarwal,Vahid Esmaeili,Abdolhossein Shahverdi +6 more
TL;DR: Current freezing techniques and novel strategies that have been developed for sperm protection against cryo-damage are discussed, as well as evaluating the probable effects of sperm freezing on offspring health.
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The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence
TL;DR: The physical and biochemical factors of cryopreservation that could potentially affect genomic integrity are described in detail to highlight the gaps in knowledge not only for clinical studies, but also for basic research in human embryos.
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Utility of antioxidants during assisted reproductive techniques : an evidence based review
TL;DR: The current body of evidence as a whole suggests the need for further well-designed and larger scale randomized controlled studies, as well as research to minimize oxidative stress conditions in the clinical ART setting.
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Effect of freezing–thawing process and quercetin on human sperm survival and DNA integrity
Nassira Zribi,Nozha Chakroun,Fatma Ben Abdallah,Henda Elleuch,Afifa Sellami,Jalel Gargouri,Tarek Rebai,Faiza Fakhfakh,Leila Keskes +8 more
TL;DR: Cryopreservation led to a significant increase in sperm DNA fragmentation, DNA oxidation and caspase 3 activation, and Supplementation of the cryopreserved medium with quercetrin induced a significant improvement in post thaw sperm parameters, compared to those of control, regarding sperm motility, viability and DNA integrity.
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Sperm DNA Fragmentation: A New Guideline for Clinicians.
Ashok Agarwal,Ahmad Majzoub,Ahmad Majzoub,Saradha Baskaran,Manesh Kumar Panner Selvam,Chak-Lam Cho,Chak-Lam Cho,Ralf Henkel,Ralf Henkel,Renata Finelli,Kristian Leisegang,Pallav Sengupta,Catalina Barbarosie,Neel Parekh,Marco G. Alves,Edmund Y. Ko,Mohamed Arafa,Mohamed Arafa,Nicholas N. Tadros,Ranjith Ramasamy,Parviz Kavoussi,Rafael F. Ambar,Manish Kuchakulla,Kathy Amy Robert,Concetta Iovine,Damayanthi Durairajanayagam,Sunil Jindal,Rupin Shah +27 more
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References
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Journal ArticleDOI
The causes of reduced fertility with cryopreserved semen
TL;DR: Evidence for new approaches for improving the performance of cryopreserved semen is offered and factors affecting the proportion of survivors and functional status of survivors are reviewed.
Journal ArticleDOI
Basic aspects of frozen storage of semen
TL;DR: The possible roles of cryoprotectants and additives are considered in the context of their putative interactions with the sperm plasma membrane and modern approaches to the laboratory assessment of spermatozoa after freeze-thawing are discussed.
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Ramadan A Saleh,Ashok Agarwal +1 more
TL;DR: Extensive research in the Cleveland Clinic indicates that the seminal oxidative stress test has diagnostic and prognostic capabilities beyond those of conventional tests of sperm quality or functions and may be of particular importance to the future management of male infertility.
Journal ArticleDOI
Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta-analysis
TL;DR: Sperm DNA damage is associated with a significantly increased risk of pregnancy loss after IVF and ICSI and these data provide a clinical indication for the evaluation of sperm DNA damage prior to IVF or I CSI and a rationale for further investigating the association between sperm DNADamage and pregnancy loss.
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TL;DR: The mitochondrial response to pro‐apoptotic stimuli is identified as a selective process leading to a hierarchical ordering of the effectors involved in cell death induction and EndoG and AIF seem to define a ‘caspase‐dependent’ mitochondria‐initiated apoptotic DNA degradation pathway that is conserved between mammals and nematodes.