Journal ArticleDOI
Identification of a transformation-specific antigen induced by an avian sarcoma virus.
TLDR
The identification of a 60,000-MW transformation-specific antigen detectable in ASV-transformed chicken cells andASV-induced hamster tumour cells is described by immunoprecipitation of radiolabelled cell extracts with serum from tumour-bearing rabbits.Abstract:
GENETIC analyses of avian sarcoma viruses (ASV) have led to the identification of a gene, designated src, which encodes a product required for the initiation and maintenance of neoplastic transformation in infected fibroblasts1–5. Because the src gene product has not been identified biochemically, this study was initiated to detect a transformation-specific protein, using serum from rabbits bearing ASV-induced tumours. We describe here the identification of a 60,000-MW transformation-specific antigen detectable in ASV-transformed chicken cells and ASV-induced hamster tumour cells by immunoprecipitation of radiolabelled cell extracts with serum from tumour-bearing rabbits. Moreover, the expression of this antigen is temperature dependent in chicken cells transformed by an ASV temperature-sensitive mutant in the src gene. The use of this antiserum may lead to the unequivocal identification and characterisation of the ASV src gene product and this, in turn, may lead to the elucidation of the mechanism of ASV-induced oncogenesis.read more
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Journal ArticleDOI
Cellular functions regulated by src family kinases
Sheila M. Thomas,Joan S. Brugge +1 more
TL;DR: This chapter reviews the evidence implicating Src family kinases in specific receptor pathways and describes the mechanisms leading to their activation, the targets that interact with these kinases, and the biological events that they regulate.
Journal ArticleDOI
Transforming gene product of Rous sarcoma virus phosphorylates tyrosine
TL;DR: It is inferred that pp60src is a novel protein kinase and that the modification of proteins via the phosphorylation of tyrosine is essential to the malignant transformation of cells by Rous sarcoma virus.
Journal ArticleDOI
Cellular Oncogenes and Multistep Carcinogenesis
TL;DR: Two dozen cellular proto-oncogenes have been discovered to date through the study of retroviruses and the use of gene transfer, and recent work provides experimental strategies by which many of them, as well as oncogene of DNA tumor viruses, may be placed into functional categories.
Journal ArticleDOI
The HSP90 chaperone machinery
TL;DR: Owing to the importance of HSP90 in the regulation of many cellular proteins, it has become a promising drug target for the treatment of several diseases, which include cancer and diseases associated with protein misfolding.
Journal ArticleDOI
Protein kinase activity associated with the avian sarcoma virus src gene product.
TL;DR: The expression of the protein kinase activity (ATP:protein phosphotransferase, EC 2.7.1.37) was growth temperature-dependent in cells infected with an ASV mutant temperature-sensitive for the transformation, and this activity was discussed in terms of protein phosphorylation as a mechanism for viral transformation.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal Article
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
TL;DR: Bacterial adsorbent not only had a distinct advantage in speed of antigen isolation, but analyses by polyacrylamide gel electrophoresis in SDS also revealed consistently higher antigen recoveries, lower levels of background radioactivity, and an absence of other cell components which may nonspecifically bind to and complicate analyses using conventional immune precipitates.
Journal ArticleDOI
Rous Sarcoma Virus: a Function required for the Maintenance of the Transformed State
TL;DR: A temperature sensitive RNA tumour virus mutant has been isolated whose properties indicate that part of the viral genome is required for transformation but not for growth.
Journal ArticleDOI
Regulation of tumor antigen synthesis by simain virus 40 gene A.
TL;DR: The presence of the same virus-induced protein in infected cells from different species and the altered behavior of that protein in mutant infection strongly suggest that the protein is virus coded.