Journal ArticleDOI
Identification of the cellular receptor for anthrax toxin.
Kenneth A. Bradley,Jeremy Mogridge,Jeremy Mogridge,Michael Mourez,Robert J. Collier,John A. Young +5 more
Reads0
Chats0
TLDR
The cloning of the human PA receptor is described using a genetic complementation approach and a soluble version of this domain can protect cells from the action of the toxin.Abstract:
The tripartite toxin secreted by Bacillus anthracis, the causative agent of anthrax, helps the bacterium evade the immune system and can kill the host during a systemic infection. Two components of the toxin enzymatically modify substrates within the cytosol of mammalian cells: oedema factor (OF) is an adenylate cyclase that impairs host defences through a variety of mechanisms including inhibiting phagocytosis; lethal factor (LF) is a zinc-dependent protease that cleaves mitogen-activated protein kinase kinase and causes lysis of macrophages. Protective antigen (PA), the third component, binds to a cellular receptor and mediates delivery of the enzymatic components to the cytosol. Here we describe the cloning of the human PA receptor using a genetic complementation approach. The receptor, termed ATR (anthrax toxin receptor), is a type I membrane protein with an extracellular von Willebrand factor A domain that binds directly to PA. In addition, a soluble version of this domain can protect cells from the action of the toxin.read more
Citations
More filters
Patent
Seneca valley virus (svv) cellular receptor targeted oncotherapy
TL;DR: In this paper, a method for selecting cancer patient for treatment with Seneca Valley Virus (SVV) by determining expression of ANTXR1 in a cancerous tissue in cancer patient; and designating the cancer patient as a candidate for treatment of SVV if normal levels or elevated levels of AN TXR1 expression is detected in the cancerous tissues.
Journal ArticleDOI
Vascular neuroeffector mechanisms: the next 30 years.
TL;DR: The 10th International Symposium on Vascular Neuroeffector Mechanisms was held on 12-15 July 2002 in Lake Tahoe, California, USA.
Journal ArticleDOI
A comparative analysis of secreted protein disulfide isomerases from the tropical co-endemic parasites Schistosoma mansoni and Leishmania major.
Adriana E. Miele,Sofiane Badaoui,Lorenzo Maugliani,Lorenzo Maugliani,Romain Salza,Giovanna Boumis,Silvia Chichiarelli,Bertrand Duclos,Sylvie Ricard-Blum +8 more
TL;DR: A previously undisclosed moonlighting behaviour of both PDIs is highlighted, namely a concentration-dependent switch of function from thiol-oxidoreductase to holdase, and the differences are exploited to look for possible compounds able to interfere with the redox activity of bothPDI.
Patent
Immunoadhesin comprising a chimeric ICAM-1 molecule produced in a plant
TL;DR: The chimeric ICAM molecule is a fusion protein that has a rhinovirus receptor protein linked to an immunoglobulin protein and may optionally also contain J chain and secretory compounds as mentioned in this paper.
References
More filters
Journal ArticleDOI
Genes expressed in human tumor endothelium
Brad St. Croix,Carlo Rago,Carlo Rago,Victor E. Velculescu,Giovanni Traverso,Katharine E. Romans,Elizabeth A. Montgomery,Anita Lal,Gregory J. Riggins,Christoph Lengauer,Bert Vogelstein,Bert Vogelstein,Kenneth W. Kinzler +12 more
TL;DR: It is demonstrated that tumor and normal endothelium are distinct at the molecular level, a finding that may have significant implications for the development of anti-angiogenic therapies.
Journal ArticleDOI
Clonal selection and learning in the antibody system
TL;DR: A second selection process occurs during immune responses in which a new antibody repertoire is generated through somatic hypermutation, where only mutants binding antigen with high affinity survive to become memory cells.
Journal ArticleDOI
Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells
TL;DR: The ability to concentrate vesicular stomatitis virus G glycoprotein pseudotyped vectors will facilitate gene therapy model studies and other gene transfer experiments that require direct delivery of vectors in vivo, and facilitate genetic studies in nonmammalian species, including the important zebrafish developmental system.
Journal ArticleDOI
Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization.
TL;DR: The successful isolation of these prepuberal cell types was accomplished by defining distinctive morphological characteristics of the cells and assessing the identity and purity of the isolated cell types by microscopy.