Journal ArticleDOI
Immobilization and characterization of porcine pancreas lipase
K. Bagi,L.M. Simon,B. Szajáni +2 more
TLDR
Porcine pancreas lipase (triacylglycerol ester hydrolase, EC 3.1.3) was immobilized with the highest activity on polyacrylamide beads possessing carboxylic functional groups activated by a water-soluble carbodiimide to stabilized the enzyme against heat and urea treatment.About:
This article is published in Enzyme and Microbial Technology.The article was published on 1997-05-15. It has received 94 citations till now. The article focuses on the topics: Immobilized enzyme & Lipase.read more
Citations
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Journal ArticleDOI
Overview of the Stability of α-Chymotrypsin in Different Solvent Media
Awanish Kumar,Pannuru Venkatesu +1 more
Journal ArticleDOI
Properties and biotechnological applications of porcine pancreatic lipase
TL;DR: PPL has high stability and activity in anhydrous media as demonstrated in esterification and transesterification reactions, and immobilization techniques which improve its catalytic activity, storage, operational, and thermal-stability properties are briefly discussed.
Journal ArticleDOI
Parameters Affecting the Performance of Immobilized Enzyme
TL;DR: This review focuses on the current status of enzyme immobilization, which aims to summarize the latest research on the parameters affecting the performance of immobilized enzyme.
Journal ArticleDOI
Protein engineering by random mutagenesis and structure-guided consensus of Geobacillus stearothermophilus Lipase T6 for enhanced stability in methanol.
TL;DR: It is suggested that the Q185L substitution facilitates a closed lid conformation that limits access for both the methanol and substrate excess into the active site, which makes this variant a potential biocatalyst for biodiesel production.
Journal ArticleDOI
Stability of responsive polymer-protein bioconjugates
TL;DR: Different ways of R PPB synthesis, types of stabilities of RPPB, factors affecting them and various strategies to enhance the stability are highlighted.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Enzyme-catalyzed processes in organic solvents.
TL;DR: Lipases can catalyze several other processes in organic media including esterification, aminolysis, acyl exchange, thiotransesterification, and oximolysis; some of these reactions proceed to an appreciable extent only in nonaqueous solvents.
Book ChapterDOI
Covalent coupling methods for inorganic support materials.
TL;DR: In this article, the covalent attachment of enzymes to organic supports is discussed, and derivatives of alkylamines and coupling techniques are described in the chapter, including aqueous and organic silanization.
Journal ArticleDOI
Purification from porcine pancreas of two molecular species with lipase activity
TL;DR: The two porcine lipases appear to be very closely related with regard to amino acid composition, molecular weight and specific activity on long- or short-chain triglycerides.