Journal ArticleDOI
Immobilization of whole Escherichia coli containing penicillin amidase using cross-linking agents and fillers
P. S. R. Babu,T. Panda +1 more
TLDR
To obtain a catalyst with good mechanical stress stability and other operational characteristics, cross-linked aggregates of whole Escherichia coli containing penicillin amidase were reinforced with surface modified precipitated silica and chitosan.Abstract:
To obtain a catalyst with good mechanical stress stability and other operational characteristics, cross-linked aggregates of whole Escherichia coli containing penicillin amidase were reinforced with surface modified precipitated silica and chitosan. The immobilized cells plus mixed fillers possess better performance characteristics i.e. higher stability at 4°C and 30°C, least protein leaching capacity and good settling characteristics. Most suitable conditions to prepare catalyst with mixed fillers were: chitosan, 0.3: silica, 0.2 g/g d.wt cell; and minimum moisture content in catalyst, 30% (w/w).read more
Citations
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Journal ArticleDOI
Immobilization of cell-associated enzymes by entrapment in polymethacrylamide beads
TL;DR: The immobilized E. coli cells containing penicillin G acylase possessed a higher activity than that reported in the literature and the mmobilized mycelia of Aspergillus sp.
Journal ArticleDOI
Immobilization of whole-cell penicillin G acylase by entrapping within polymethacrylamide beads.
TL;DR: A kinetic model, in which the mass transfer resistances as a result of external film mass transfer and pore diffusion were assumed to be negligible, could properly describe the hydrolysis of penicillin G by the cells entrapped within the polymethacylamide beads.
Journal ArticleDOI
Comparative analysis of different whole cell immobilized aspergillus niger catalysts for gluconic acid fermentation using pretreated cane molasses
D. Subba Rao,Tapobrata Panda +1 more
TL;DR: A comparative analysis of yield has revealed that calcium alginate entrapment was the most suitable technique as it had given the maximum product yield (0.40 g gluconic acid/g total reducing sugar supplied).
Journal ArticleDOI
Immobilization of Cell-Associated Enzyme by Entrapping in Gluten Matrix
TL;DR: In this work, a procedure for immobilization of cell-associated enzymes by entrapping within gluten matrices was developed and it was found that the gel matrices hardened by the former were more accessible to the substrate and less harmful to the enzyme.
Journal ArticleDOI
Chitosan-Based Membranes as Gentamicin Carriers for Biomedical Applications—Influence of Chitosan Molecular Weight
Milena Supernak,Balbina Makurat-Kasprolewicz,Beata Kaczmarek-Szczepańska,Anna Pałubicka,Monika Sakowicz-Burkiewicz,Anna Ronowska,Marcin Wekwejt +6 more
TL;DR: In this paper , the effect of various chitosan characteristics on its ability to carry different antibiotics is discussed in the literature, and the influence of different molecular weights of this polymer on its potential as an antibacterial membrane after adding gentamicin (1% w/w).
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book ChapterDOI
Immobilized Living Cells and Their Applications
TL;DR: Immobilized living cells should be preferred to immobilized enzymes for degradative and synthetic reactions in industry; another major advantage of immobilized cells is that the operational stability of the immobilizedliving cells may often be greatly enhanced by the regeneration of the enzyme activities of the inhibitor cells.
Journal ArticleDOI
Production of 6-aminopenicillanic acid by immobilized Pleurotus ostreatus
TL;DR: 6-Aminopenicillanic acid from penicillin V is produced by Pleurotus ostreatus immobilized by entrapment in a chitosan matrix in carriers in which the cell concentration increases after network formation by irreversible shrinking of the biocatalyst.
Related Papers (5)
Studies on improved techniques for immobilizing and stabilizing penicillin amidase associated with E. coli cells
P.S.R. Babu,Tapobrata Panda +1 more