Open Access
Improving plant drought, salt and freezing tolerance by gene transfer of a single stress-inducible transcription factor
Kazuko Yamaguchi-Shinozaki,Kazuo Shinozaki +1 more
- Vol. 236, pp 176-189
TLDR
It is shown here that overexpression of the cDNA encoding DREB1A in transgenic plants activated the expression of many of these stress tolerance genes under normal growing conditions and resulted in improved tolerance to drought, salt loading, and freezing.Abstract:
Plant productivity is greatly affected by environmental stresses such as drought, salt loading and freezing. We reported that a cis-acting promoter element, the dehydration response element (DRE), plays an important role in regulating gene expression in response to these stresses in Arabidopsis. The transcription factor DREB1A specifically interacts with the DRE and induces expression of stress tolerance genes. We show here that overexpression of the cDNA encoding DREB1A in transgenic Arabidopsis plants activated the expression of many of theses stress tolerance genes under normal growing conditions and resulted in improved tolerance to drought, salt loading and freezing. However, use of the strong constitutive 35S cauliflower mosaic virus (CaMV) promoter to drive expression of DREB1A also resulted in severe growth retardation under normal growing conditions. In contrast, expression of DREB1A from the stress-inducible rd29A promoter gave rise to minimal effects on plant growth while providing an even greater tolerance to stress conditions than did expression of the gene from the CaMV promoter. As the DRE-related regulatory element is not limited to Arabidopsis the DREB1A cDNA and the rd29A promoter may be useful for improving the stress tolerance of agriculturally important crops by gene transfer.read more
Citations
More filters
Journal ArticleDOI
Salt and drought stress signal transduction in plants
TL;DR: Salt and drought stress signal transduction consists of ionic and osmotic homeostasis signaling pathways, detoxification (i.e., damage control and repair) response pathways, and pathways for growth regulation.
Journal ArticleDOI
Plant cellular and molecular responses to high salinity.
TL;DR: Evidence for plant stress signaling systems is summarized, some of which have components analogous to those that regulate osmotic stress responses of yeast, some that presumably function in intercellular coordination or regulation of effector genes in a cell-/tissue-specific context required for tolerance of plants.
Journal ArticleDOI
Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance
TL;DR: The present review summarizes the recent advances in elucidating stress-response mechanisms and their biotechnological applications and examines the following aspects: regulatory controls, metabolite engineering, ion transport, antioxidants and detoxification, late embryogenesis abundant (LEA) and heat-shock proteins.
Journal ArticleDOI
Plant salt tolerance
TL;DR: A recently discovered halophytic plant species, Thellungiella halophila, now promises to help in the detection of new tolerance determinants and operating pathways in a model system that is not limited to Arabidopsis traits or ecotype variations.
Journal ArticleDOI
Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses.
TL;DR: This review article highlights transcriptional regulation of gene expression in response to drought and cold stresses, with particular emphasis on the role of transcription factors and cis-acting elements in stress-inducible promoters.
References
More filters
Journal ArticleDOI
Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis.
Qiang Liu,Mie Kasuga,Yoh Sakuma,Hiroshi Abe,Setsuko Miura,Kazuko Yamaguchi-Shinozaki,Kazuo Shinozaki +6 more
TL;DR: Overexpression of the DREB1A cDNA in transgenic Arabidopsis plants not only induced strong expression of the target genes under unstressed conditions but also caused dwarfed phenotypes in the transgenic plants, and revealed freezing and dehydration tolerance.
Journal ArticleDOI
A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress.
TL;DR: The rd29A promoter in both transgenic Arabidopsis and tobacco was analyzed and a novel cis-acting, dehydration-responsive element (DRE) containing 9 bp, TACCGACAT, was identified that is involved in the first rapid response of rd 29A to conditions of dehydration or high salt.
Journal ArticleDOI
Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit
TL;DR: It is concluded that CBF1 can function as a transcriptional activator that binds to the C-repeat/DRE DNA regulatory element and, thus, is likely to have a role in cold- and dehydration-regulated gene expression in Arabidopsis.
Journal ArticleDOI
Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance.
Kirsten Jaglo-Ottosen,Sarah J. Gilmour,Daniel Zarka,Oliver Schabenberger,Michael F. Thomashow +4 more
TL;DR: Increased expression of Arabidopsis CBF1, a transcriptional activator that binds to the CRT/DRE sequence, induced COR gene expression and increased the freezing tolerance of nonacclimatedArabidopsis plants.
Journal ArticleDOI
Overexpression of [delta]-Pyrroline-5-Carboxylate Synthetase Increases Proline Production and Confers Osmotolerance in Transgenic Plants
TL;DR: Proline (Pro) accumulation has been correlated with tolerance to drought and salinity stresses in plants and overproduction of Pro in plants may lead to increased tolerance against these abiotic stresses, suggesting that activity of the first enzyme of the pathway is the rate-limiting factor in Pro synthesis.