Patent
In vitro induction of dopaminergic cells
Samuel Weiss,Brent A. Reynolds +1 more
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In this paper, the expression of thyrosine hydroxylase in neural cells in vitro can be induced by contacting neural cells with a culture medium comprising at least one member of the fibroblast growth factor family in combination with conditioned medium or molecules of the transforming growth factor beta family.Abstract:
The expression of thyrosine hydroxylase in neural cells in vitro can be induced by contacting neural cells with a culture medium comprising at least one member of the fibroblast growth factor family in combination with conditioned medium or molecules of the transforming growth factor beta family. The method induces neural cells obtained from normally non-dopaminergic neural tissue such as the striatum and the cortex to express tyrosine hydroxylase. The cells can be used to treat neurological disorders in patients requiring dopaminergic cells.read more
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References
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Journal ArticleDOI
Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system
Brent A. Reynolds,Samuel Weiss +1 more
TL;DR: Cells of the adult mouse striatum have the capacity to divide and differentiate into neurons and astrocytes.
Journal ArticleDOI
CNS stem cells express a new class of intermediate filament protein.
TL;DR: The predicted amino acid sequence of the nestin gene product shows that nestin defines a distinct sixth class of intermediate filament protein, extending a model in which transitions in intermediate filament gene expression reflect major steps in the pathway of neural differentiation.
Journal ArticleDOI
GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons
TL;DR: In embryonic midbrain cultures, recombinant human GDNF promoted the survival and morphological differentiation of dopaminergic neurons and increased their high-affinity dopamine uptake and did not increase total neuron or astrocyte numbers or transmitter uptake.
Journal ArticleDOI
A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes.
TL;DR: It is suggested that EGF and/or TGF alpha may act on a multipotent progenitor cell in the striatum to generate both neurons and astrocytes.
Journal ArticleDOI
BDNF is a neurotrophic factor for dopaminergic neurons of the substantia nigra.
Carolyn Hyman,Magdalena Hofer,Yves-Alain Barde,M Juhasz,George D. Yancopoulos,Stephen P. Squinto,Ronald M. Lindsay +6 more
TL;DR: BRAIN-derived neurotrophic factor seems to be a trophic factor for mesencephalic dopaminergic neurons, increasing their survival, including that of neuronal cells which degenerate in Parkinson's disease.