Journal ArticleDOI
In vitro regeneration of silktree (Albizzia julibrissin) from excised roots
TLDR
Higher concentrations of benzyladenine and Zeatin were required to enhance shoot formation in silktree, compared to TDZ, and thidiazuron was highly effective in stimulating shoot formation at low concentrations (<1 μM.Abstract:
Root segments (1 cm long) were excised from 15–20 day old seedlings of silktree (Albizzia julibrissin) grown on B5 medium. About 50% of the control (no growth regulators added) root explants formed shoot buds within 15 days after placement on the culture medium. After 30 days, there were about 4 shoots per control explant. Addition of low levels of various auxins (0.5 μM) did not influence the formation of shoot buds from the explants. Higher concentrations (5μM), however, decreased shoot regeneration. Kinetin and 2iP did not influence shoot regeneration at the concentrations tested (1 & 10 μM). Addition of benzyladenine, Zeatin, or thidiazuron to the culture medium increased both the percentage of explants that formed shoots and the number of shoots per explant. Thidiazuron was highly effective in stimulating shoot formation at low concentrations (<1 μM). At 0.05 μM thidiazuron, 95% of the explants produced shoots and about 10 shoots were formed per explant. Compared to TDZ, higher concentrations (10 μM) of benzyladenine and Zeatin were required to enhance shoot formation. Upon excision and transfer to B5 medium, regenerated shoots developed into normal rooted plantlets.read more
Citations
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Journal ArticleDOI
Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview
TL;DR: The present communication attempts to overview the progress in tissue culture, genetic transformation and biotechnological applications in the last decade and future implications.
Journal ArticleDOI
In vitro differentiation and plant regeneration of albizia chinensis (osb.) merr.
TL;DR: Petiolar and distal cotyledonary segments of Albizia chinensis were cultured on Murashige and Skoog's medium and induced to form adventitious shoot buds in the presence of either cytokinins 6-benzylamino purine (BAP), kinetin (KN) or thidiazuron (TDZ).
Journal ArticleDOI
Genetic transformation of silver birch (Betula pendula) by particle bombardment
TL;DR: Clonal variation in transformation efficiency was found in transient expression of the beta-glucuronidase gene in callus cultures and in plantlets transformed by stable integration of the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit and neomycin phosphotransferase (npt2) genes.
Journal ArticleDOI
Plant regeneration, origin, and development of shoot buds from root segments of melia azedarach l. (meliaceae) seedlings
TL;DR: Histological analysis of roots from intact seedlings (without treatment) demonstrated that during the early life of the roots, M. azedarach lacks preformed buds, and when the roots were excised and cultured in vitro, the histology and SEM observations revealed that buds originated from meristematic groups of cells, which had been formed from the pericycle and several layers beneath.
Journal ArticleDOI
Stimulation of Shoot Regeneration on Linum Hypocotyl Segments by Thidiazuron and Its Response to Light and Calcium
TL;DR: A drastic reduction in regeneration response on hormone-free as well as TDZ-supplemented medium was found after inclusion of an inhibitor of calcium-uptake, lanthanum (La3+).
References
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Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI
Thidiazuron: a potent cytokinin for woody plant tissue culture
Carl A. Huetteman,John E. Preece +1 more
TL;DR: Thidizuron (TDZ) is among the most active cytokinin-like substances for woody plant tissue culture and facilitates efficient micropropagation of many recalcitrant woody species.
Journal ArticleDOI
Cytokinin activity induced by thidiazuron.
TL;DR: Analysis of soybean callus extracts strongly suggests that thidiazuron treatment creates an accumulation and/or synthesis of purine cytokinins, able to induce the growth, expansion and regeneration, mentioned above.
Journal Article
Biological and biochemical effects of cytokinin-active phenylurea derivatives in tissue culture systems
Journal ArticleDOI
Induction by thidiazuron of somatic embryogenesis in intact seedlings of peanut
TL;DR: Thidiazuron-induced direct embryogenesis from morphologically intact seedlings may provide an excellent experimental system for investigating somatic embryogenesis and the morphoregulatory role of TDZ.
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