In vivo imaging of neural activity
Weijian Yang,Rafael Yuste +1 more
TLDR
Advanced microscopy techniques for in vivo functional imaging are reviewed and guidelines for which technologies are best suited for particular applications are offered.Abstract:
Since the introduction of calcium imaging to monitor neuronal activity with single-cell resolution, optical imaging methods have revolutionized neuroscience by enabling systematic recordings of neuronal circuits in living animals. The plethora of methods for functional neural imaging can be daunting to the nonexpert to navigate. Here we review advanced microscopy techniques for in vivo functional imaging and offer guidelines for which technologies are best suited for particular applications.read more
Citations
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Neuronal cell-type classification: challenges, opportunities and the path forward
Hongkui Zeng,Joshua R. Sanes +1 more
TL;DR: In this paper, a staged approach for cell type classification in the brain is proposed, including the incorporation of multiple, quantitative features as criteria, the use of discontinuous variation to define types and the creation of a hierarchical system to represent relationships between cells.
Journal ArticleDOI
Near-Infrared-II Molecular Dyes for Cancer Imaging and Surgery.
TL;DR: This review focuses on summarizing organic dyes emitting at a biological transparency window termed the near‐infrared‐II (NIR‐II) window, where minimal light interaction with the surrounding tissues allows photons to travel nearly unperturbed throughout the body.
Journal ArticleDOI
Linking Structure and Function in Macroscale Brain Networks.
TL;DR: The current state of knowledge linking structure and function in macroscale brain networks is synthesized and it is argued that current models do not include the requisite biological detail to completely predict function.
Journal ArticleDOI
Adaptive optical fluorescence microscopy
TL;DR: Recent work on incorporating adaptive optics, a technology originally applied in astronomical telescopes to combat atmospheric aberrations, to improve image quality of fluorescence microscopy for biological imaging is reviewed.
Journal ArticleDOI
Novel electrode technologies for neural recordings.
Guosong Hong,Charles M. Lieber +1 more
TL;DR: It is described how these emergent neurotechnologies can approach the ultimate goal of illuminating chronic brain activity with minimal disruption of the neural environment, thereby providing unprecedented opportunities for neuroscience research in the future.
References
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Fritjof Helmchen,Winfried Denk +1 more
TL;DR: Fundamental concepts of nonlinear microscopy are reviewed and conditions relevant for achieving large imaging depths in intact tissue are discussed.
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Nonlinear magic: multiphoton microscopy in the biosciences
TL;DR: Multiphoton microscopy has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals and its use is now increasing exponentially.