Induction of Beige-Like Adipocytes in 3T3-L1 Cells
Hiroki Asano,Yohei Kanamori,Satoshi Higurashi,Takayuki Y. Nara,Ken Kato,Tohru Matsui,Masayuki Funaba +6 more
TLDR
In this article, the authors explored the factors responsible for the differentiation of 3T3-L1 white preadipocytes to beige adipocytes and found that the gene expression profile of the beige-selective genes in the adipocytes induced by the mixture of T3, IBMX and Rosi did not differ from those in the control cells.Abstract:
There are two types of brown adipocytes: classical brown adipocytes that form the brown fat depots and beige adipocytes that emerge in the white fat depots. Beige adipocytes have a low level of uncoupling protein 1 (Ucp1) expression in the basal state, but Ucp1 expression is increased in response to β adrenergic receptor activation. The present study explored the factors responsible for the differentiation of 3T3-L1 white preadipocytes to beige adipocytes. Significant expression of Ucp1 was not detected under any tested conditions in the absence of isoproterenol (Iso), an agonist of β adrenergic receptor. Iso-induced Ucp1 expression was significantly higher in the cells treated with a mixture of triiodothyronine (T3) and 3-isobutyl-1-methylxanthine (IBMX) for days 0–8 than in the control cells. Chronic IBMX treatment was indispensable for the enhanced Iso-induced Ucp1 expression, and treatment with additional rosiglitazone (Rosi) for days 0–8 further increased the Ucp1 expression. Recently, genes were identified that are predominantly expressed in beige adipocytes, which were induced from stromal vascular cells in white fat depots. However, the expression levels of the beige adipocyte-selective genes in the adipocytes induced by the mixture of T3, IBMX and Rosi did not differ from those in the control adipocytes. The present study indicates that 3T3-L1 cells can differentiate to beige-like adipocytes by prolonged treatment with the mixture of T3, IBMX and Rosi and that the gene expression profile of the adipocytes is distinct from those previously induced from white fat depots.read more
Citations
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Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review.
Francisco Javier Ruiz-Ojeda,Azahara I. Rupérez,Carolina Gomez-Llorente,Angel Gil,Concepción M. Aguilera +4 more
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BAR502, a dual FXR and GPBAR1 agonist, promotes browning of white adipose tissue and reverses liver steatosis and fibrosis
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TL;DR: BAR502, a dual FXR and GPBAR1 agonist, protects against liver damage caused by HFD by promoting the browning of adipose tissue.
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Quercetin, a functional compound of onion peel, remodels white adipocytes to brown-like adipocytes
TL;DR: This study provides the first demonstration of the browning effects of onion peel and quercetin using both animal and cell models and indicates that onion peel has the potential to remodel the characteristics of white adipocytes to those of brown-like adipocytes.
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Ginsenoside Rb1 promotes browning through regulation of PPARγ in 3T3-L1 adipocytes.
Qianqian Mu,Xin Fang,Xiao-Ke Li,Dandan Zhao,Fangfang Mo,Guangjian Jiang,Na Yu,Yi Zhang,Yubo Guo,Min Fu,Jun-Li Liu,Dongwei Zhang,Sihua Gao +12 more
TL;DR: Ginsenoside Rb1 is suggested to promote browning of 3T3-L1 adipocytes through induction of PPARγ, a new source to discover browning agonists and also useful to understand and extend the applications of ginseng and its constituents.
Journal ArticleDOI
MiR-494-3p regulates mitochondrial biogenesis and thermogenesis through PGC1-α signalling in beige adipocytes
Mengistu Lemecha,Katsutaro Morino,Takeshi Imamura,Hirotaka Iwasaki,Natsuko Ohashi,Shogo Ida,Daisuke Sato,Osamu Sekine,Satoshi Ugi,Hiroshi Maegawa +9 more
TL;DR: It is demonstrated that decreased miR-494-3p expression during browning regulates mitochondrial biogenesis and thermogenesis through PGC1-α.
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