Inertio-elastic focusing of bioparticles in microchannels at high throughput
Eugene J. Lim,Thomas Joseph Ober,Jon F. Edd,Salil P. Desai,Douglas Neal,Ki Wan Bong,Patrick S. Doyle,Gareth H. McKinley,Mehmet Toner +8 more
TLDR
A hydrodynamic approach is reported that enables deterministic focusing of beads, mammalian cells and anisotropic hydrogel particles in a microchannel at extremely high flow rates and demonstrates bioparticle focusing at flow rates that are the highest yet achieved.Abstract:
Controlled manipulation of particles from very large volumes of fluid at high throughput is critical for many biomedical, environmental and industrial applications. One promising approach is to use microfluidic technologies that rely on fluid inertia or elasticity to drive lateral migration of particles to stable equilibrium positions in a microchannel. Here, we report on a hydrodynamic approach that enables deterministic focusing of beads, mammalian cells and anisotropic hydrogel particles in a microchannel at extremely high flow rates. We show that on addition of micromolar concentrations of hyaluronic acid, the resulting fluid viscoelasticity can be used to control the focal position of particles at Reynolds numbers up to Re≈10,000 with corresponding flow rates and particle velocities up to 50 ml min−1 and 130 m s−1. This study explores a previously unattained regime of inertio-elastic fluid flow and demonstrates bioparticle focusing at flow rates that are the highest yet achieved. Controlled manipulation of particles from very large volumes of fluid at high throughput is critical for many real-world applications. Here, the authors show bioparticle focusing in a microchannel for a previously unattained regime of inertio-elastic flow at Reynolds numbers up to 10,000.read more
Citations
More filters
Journal ArticleDOI
Fundamentals and applications of inertial microfluidics: a review
Jun Zhang,Sheng Yan,Dan Yuan,Gursel Alici,Nam-Trung Nguyen,Majid Ebrahimi Warkiani,Weihua Li +6 more
TL;DR: This review discusses the fundamental kinematics of particles in microchannels to familiarise readers with the mechanisms and underlying physics in inertial microfluidic systems and presents a comprehensive review of recent developments and key applications of inertialMicrofluidics systems according to their microchannel structures.
Journal ArticleDOI
Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows.
Chao Liu,Jiayi Guo,Jiayi Guo,Fei Tian,Na Yang,Fusheng Yan,Yanping Ding,Jingyan Wei,Guoqing Hu,Guangjun Nie,Jiashu Sun +10 more
TL;DR: A viscoelasticity-based microfluidic system to directly separate exosomes from cell culture media or serum in a continuous, size-dependent, and label-free manner is presented and may serve as a versatile platform to facilitate exosome analyses in diverse biochemical applications.
Journal ArticleDOI
Particle manipulations in non-Newtonian microfluidics: A review.
TL;DR: An overview of the various passive manipulations, including focusing, separation, washing and stretching, of particles that have thus far been demonstrated in non-Newtonian microfluidics.
Journal ArticleDOI
Recent progress of particle migration in viscoelastic fluids
TL;DR: A comprehensive review on the applications of viscoelasticity-induced particle migration (particle separation, cell deformability measurement and alignment, particle solution exchange, rheometry-on-a-chip and others) is presented.
Journal ArticleDOI
Particle Migration due to Viscoelasticity of the Suspending Liquid and Its Relevance in Microfluidic Devices
TL;DR: In this paper, the authors present the current understanding, both from experiments and theoretical analysis, of viscoelasticity-driven cross-flow migration in microfluidic systems.
References
More filters
Journal ArticleDOI
Science and technology for water purification in the coming decades
Mark A. Shannon,Paul W. Bohn,Paul W. Bohn,Menachem Elimelech,Menachem Elimelech,John G. Georgiadis,Benito J. Mariñas,Anne M. Mayes,Anne M. Mayes +8 more
TL;DR: Some of the science and technology being developed to improve the disinfection and decontamination of water, as well as efforts to increase water supplies through the safe re-use of wastewater and efficient desalination of sea and brackish water are highlighted.
Book
Viscous Fluid Flow
TL;DR: In this article, the stability of Laminar Boundary Layer Flow Appendices has been investigated in Cylindrical and Spherical Coordinates of Incompressible Newtonian Fluids.
Book
Particle Image Velocimetry: A Practical Guide
TL;DR: In this paper, the authors present a practical guide for the planning, performance and understanding of experiments employing the PIV technique, which is primarily intended for engineers, scientists and students, who already have some basic knowledge of fluid mechanics and nonintrusive optical measurement techniques.
Journal ArticleDOI
Isolation of rare circulating tumour cells in cancer patients by microchip technology.
Sunitha Nagrath,Lecia V. Sequist,Shyamala Maheswaran,Daphne W. Bell,Daphne W. Bell,Daniel Irimia,Lindsey Ulkus,Matthew R. Smith,Eunice L. Kwak,Subba R. Digumarthy,Alona Muzikansky,Paula D. Ryan,Ulysses J. Balis,Ulysses J. Balis,Ronald G. Tompkins,Daniel A. Haber,Mehmet Toner +16 more
TL;DR: The CTC-chip successfully identified CTCs in the peripheral blood of patients with metastatic lung, prostate, pancreatic, breast and colon cancer in 115 of 116 samples, with a range of 5–1,281CTCs per ml and approximately 50% purity.