Intracellular Distribution of Rubella Virus Nonstructural Protein P150
TLDR
Antiserum prepared against an amino-terminal fragment of rubella virus (RUB) nonstructural polyprotein was used to study RUB-infected Vero cells, indicating that these structures may be the sites of viral RNA synthesis.Abstract:
Antiserum prepared against an amino-terminal fragment of rubella virus (RUB) nonstructural polyprotein was used to study RUB-infected Vero cells. Replicase protein P150 was associated with vesicles and vacuoles of endolysosomal origin and later with large, convoluted, tubular membrane structures. Newly incorporated bromouridine was associated with the same structures and specifically with small membrane invaginations, spherules, indicating that these structures may be the sites of viral RNA synthesis.read more
Citations
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Journal ArticleDOI
Modification of intracellular membrane structures for virus replication
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A Positive-Strand RNA Virus Replication Complex Parallels Form and Function of Retrovirus Capsids
Michael P. Schwartz,Jianbo Chen,Michael Janda,Michael L. Sullivan,Johan den Boon,Paul Ahlquist +5 more
TL;DR: It is shown that brome mosaic virus RNA replication protein 1a, 2a polymerase, and a cis-acting replication signal recapitulate the functions of Gag, Pol, and RNA packaging signals in conventional retrovirus and foamy virus cores, revealing fundamental similarities in replication and may have common evolutionary origins.
Book ChapterDOI
Viral RNA replication in association with cellular membranes.
TL;DR: All plus-strand RNA viruses replicate in association with cytoplasmic membranes of infected cells, and studies of individual nonstructural proteins have revealed that the replication complexes are associated with the membranes and targeted to the respective organelle by the ns proteins rather than RNA.
Journal ArticleDOI
The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex.
TL;DR: It is shown that the virus-induced recruitment of host proteins and membrane appears to occur at a pre-Golgi step and that a majority of the viral RNA species housed within the RC is in a double-stranded RNA (dsRNA) form.
Journal ArticleDOI
Three-dimensional analysis of a viral RNA replication complex reveals a virus-induced mini-organelle.
TL;DR: The results identify spherules as the site of protein A and nascent RNA accumulation and define spherule topology, dimensions, and stoichiometry to reveal the nature and many details of the organization and function of the FHV RNA replication complex.
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