Open Access
Invited speakers; direct conversion of fibroblasts to functional neurons by defined factors
M Wernig
- Vol. 12, Iss: 16, pp 13-13
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TLDR
This work identified a combination of only three factors, Ascl1, Brn2 and Myt1l, that suffice to rapidly and efficiently convert mouse embryonic and postnatal fibroblasts into functional neurons in vitro, and induced neuronal (iN) cells express multiple neuron-specific proteins, generate action potentials and form functional synapses.About:
The article was published on 2010-01-01 and is currently open access. It has received 2332 citations till now.read more
Citations
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RNA-guided gene activation by CRISPR-Cas9-based transcription factors
Pablo Perez-Pinera,D. Dewran Kocak,Christopher M. Vockley,Andrew F. Adler,Ami M. Kabadi,Lauren R. Polstein,Pratiksha I. Thakore,Katherine A. Glass,David G. Ousterout,Kam W. Leong,Kam W. Leong,Farshid Guilak,Gregory E. Crawford,Timothy E. Reddy,Charles A. Gersbach +14 more
TL;DR: A Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules is created, demonstrating a simple and versatile approach for RNA-guided gene activation.
Journal Article
Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors
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Transcriptional Regulation and Its Misregulation in Disease
Tong Ihn Lee,Richard A. Young +1 more
TL;DR: Recent advances in understanding of transcriptional regulation are reviewed and how these have provided new insights into transcriptional misregulation in disease are discussed.
Journal ArticleDOI
Induction of human neuronal cells by defined transcription factors
Zhiping P. Pang,Nan Yang,Thomas Vierbuchen,Austin Ostermeier,Daniel R. Fuentes,Troy Q. Yang,Ami Citri,Vittorio Sebastiano,Samuele Marro,Thomas C. Südhof,Marius Wernig +10 more
TL;DR: The data demonstrate that non-neural human somatic cells, as well as pluripotent stem cells, can be converted directly into neurons by lineage-determining transcription factors.
Journal ArticleDOI
Rapid Single-Step Induction of Functional Neurons from Human Pluripotent Stem Cells
Yingsha Zhang,ChangHui Pak,Yan Han,Henrik Ahlenius,Zhenjie Zhang,Soham Chanda,Samuele Marro,Christopher Patzke,Claudio Acuna,Jason P. Covy,Wei Xu,Nan Yang,Tamas Danko,Lu Chen,Marius Wernig,Thomas C. Südhof +15 more
TL;DR: It is shown that human ESCs and iPSCs can be converted into functional iN cells with nearly 100% yield and purity in less than 2 weeks by forced expression of a single transcription factor.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Translating the Histone Code
Thomas Jenuwein,C. David Allis +1 more
TL;DR: It is proposed that this epigenetic marking system represents a fundamental regulatory mechanism that has an impact on most, if not all, chromatin-templated processes, with far-reaching consequences for cell fate decisions and both normal and pathological development.
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Expression of a single transfected cDNA converts fibroblasts to myoblasts.
TL;DR: In this article, the major open reading frame encoded by this cDNA contains a short protein segment similar to a sequence present in the myc protein family, and the expression of one of these cDNAs transfected into C3H10T1/2 fibroblasts, where it is not normally expressed, is sufficient to convert them to stable myoblasts.
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The mammalian epigenome.
TL;DR: Current research efforts are reviewed, with an emphasis on large-scale studies, emerging technologies, and challenges ahead.
Journal ArticleDOI
In vivo reprogramming of adult pancreatic exocrine cells to beta-cells.
TL;DR: This study identifies a specific combination of three transcription factors (Ngn3) Pdx1 and Mafa that reprograms differentiated pancreatic exocrine cells in adult mice into cells that closely resemble β-cells, and suggests a general paradigm for directing cell reprogramming without reversion to a pluripotent stem cell state.