Isolation and Characterization of a Thermophilic Strain of Methanosarcina Unable to Use H(2)-CO(2) for Methanogenesis.
Stephen H. Zinder,Robert A. Mah +1 more
Reads0
Chats0
TLDR
The high numbers of Methanosarcina-like clumps in sludges from thermophilic digestors and the fast generation times reported here for Methanos Arcina strain TM-1 indicate that MethanosARCina may play an important role in thermophobic methanogenesis.Abstract:
A thermophilic strain of Methanosarcina, designated Methanosarcina strain TM-1, was isolated from a laboratory-scale 55°C anaerobic sludge digestor by the Hungate roll-tube technique. Penicillin and d-cycloserine, inhibitors of peptidoglycan synthesis, were used as selective agents to eliminate contaminating non-methanogens. Methanosarcina strain TM-1 had a temperature optimum for methanogenesis near 50°C and grew at 55°C but not at 60°C. Substrates used for methanogenesis and growth by Methanosarcina strain TM-1 were acetate (12-h doubling time), methanol (7- to 10-h doubling time), methanol-acetate mixtures (5-h doubling time), methylamine, and trimethylamine. When radioactively labeled acetate was the sole methanogenic substrate added to the growth medium, it was predominantly split to methane and carbon dioxide. When methanol was also present in the medium, the metabolism of acetate shifted to its oxidation and incorporation into cell material. Electrons derived from acetate oxidation apparently were used to reduce methanol. H2-CO2 was not used for growth and methanogenesis by Methanosarcina strain TM-1. When presented with both H2-CO2 and methanol, Methanosarcina strain TM-1 was capable of limited hydrogen metabolism during growth on methanol, but hydrogen metabolism ceased once the methanol was depleted. Methanosarcina strain TM-1 required a growth factor (or growth factors) present in the supernatant of anaerobic digestor sludge. Growth factor requirements and the inability to use H2-CO2 are characteristics not found in other described Methanosarcina strains. The high numbers of Methanosarcina-like clumps in sludges from thermophilic digestors and the fast generation times reported here for Methanosarcina TM-1 indicate that Methanosarcina may play an important role in thermophilic methanogenesis.read more
Citations
More filters
Journal ArticleDOI
Biogenic methane formation in marine and freshwater environments: CO2 reduction vs. acetate fermentation—Isotope evidence
TL;DR: In this paper, the carbon and hydrogen stable isotope composition of the methane as a function of the coexisting carbon dioxide and formation water precursors is used to distinguish two primary methanogenic pathways.
Journal ArticleDOI
Anaerobic digestion of swine manure: Inhibition by ammonia
TL;DR: A stable anaerobic degradation of swine manure with ammonia concentration of 6 g-N/litre was obtained in continuously stirred tank reactors with a hydraulic retention time of 15 days, at four different temperatures as discussed by the authors.
Journal ArticleDOI
The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review
B. Demirel,Paul Scherer +1 more
TL;DR: The aim of this paper is primarily to review the recent literature about the occurrence of both acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of particulate biomass to methane (not wastewater treatment), while this review does not cover the activity of the acetate oxidizing bacteria.
Book ChapterDOI
Physiological Ecology of Methanogens
TL;DR: Biological methanogenesis plays a major role in the carbon cycle on Earth and is the terminal step in carbon flow in many anaerobic habitats, including marine and freshwater sediments, marshes and swamps, flooded soils, bogs, geothermal habitats, and animal gastrointestinal tracts as discussed by the authors.
Journal ArticleDOI
Kinetics of anaerobic treatment: A critical review
TL;DR: The fundamentals of microbial kinetics and continuous culture models are presented and the effect of temperature and inhibitors on the intrinsic kinetic rates is discussed, and Stoichiometric and bioenergetic considerations are reviewed.
References
More filters
Journal ArticleDOI
Energy conservation in chemotrophic anaerobic bacteria.
TL;DR: This article corrects the article on p. 100 in vol.
Journal ArticleDOI
New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere.
W E Balch,R S Wolfe +1 more
TL;DR: A very sensitive and precise requirement for HS-CoM in the nutrition of this fastidious anaerobe is revealed.
Journal ArticleDOI
The biology of methanogenic bacteria.
TL;DR: This article corrects the article on p. 517 in vol.
Journal ArticleDOI
Methanobacterium thermoautotrophicus sp. n., an Anaerobic, Autotrophic, Extreme Thermophile
J. G. Zeikus,R. S. Wolee +1 more
TL;DR: The isolation of a new methanogenic bacterium, Methanobacterium thermoautotrophicus sp.N.
Journal ArticleDOI
Classification of methanogenic bacteria by 16S ribosomal RNA characterization.
TL;DR: Comparative analysis of methanogens to constitute a distinct phylogenetic group containing two major divisions, and these organisms appear to be only distantly related to typical bacteria.