Open Access
Isolation, Purification and Effect on Ionic Permeability of Membranes
Gkrard Helynck,Bang Luu,J. L. Nussbaum,Douglas Picken,Georges Skalidis,Elisabeth Trifilieff,Alain Van Dorsselaer,Patrick Seta,Roger Sandeaux,Claude Gavach,Daniel Simon,Gerard Spach +11 more
TLDR
Transmembrane ion transfers were studied by 22Na + flux and electrical conductance measurements, and single channel events were observed at low protein concentrations, in particular with one the final homogeneous apoproteolipids of molecular mass 24 kDa.About:
The article was published on 1983-01-01 and is currently open access. It has received 82 citations till now. The article focuses on the topics: Membrane.read more
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Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds
TL;DR: Hydrophilic-interaction chromatography fractionations resemble those obtained through partitioning mechanisms, and the chromatography of DNA, in particular, resembles the partitioning observed with aqueous two-phase systems based on polyethylene glycol and dextran solutions.
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Premature arrest of myelin formation in transgenic mice with increased proteolipid protein gene dosage.
TL;DR: Dysmyelination of PLP transgenic mice provides experimental evidence that Pelizaeus-Merzbacher disease, previously associated with a partial duplication of the human X chromosome, can be caused by doubling of the PLP gene dosage.
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Glial cell degeneration and hypomyelination caused by overexpression of myelin proteolipid protein gene.
Tetsushi Kagawa,Kazuhiro Ikenaka,Yoshiro Inoue,Shigeki Kuriyama,Tadasu Tsujii,Junji Nakao,Kazunori Nakajima,Jun Aruga,Hideyuki Okano,Katsuhiko Mikoshiba +9 more
TL;DR: Tight regulation of Plp gene expression is necessary for normal oligodendrocyte differentiation and survival, and its overexpression can be the cause of both dys- and demyelination.
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PLP1-related inherited dysmyelinating disorders: Pelizaeus-Merzbacher disease and spastic paraplegia type 2.
TL;DR: The previous and current knowledge of the molecular pathogenesis of PMD/SPG2 is reviewed and future directions for PMD and SPG2 studies are delineated.
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Proteolipid protein (PLP) of CNS myelin: positions of free, disulfide-bonded, and fatty acid thioester-linked cysteine residues and implications for the membrane topology of PLP
Thomas Weimbs,Wilhelm Stoffel +1 more
TL;DR: The structural data not only demand the revision of the concept of the membrane topology of PLP but will also promote more sophisticated studies on the mechanism of myelination and new functions ofPLP.
References
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Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
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Modification of the Lowry procedure for the analysis of proteolipid protein.
TL;DR: A modification of the Lowry procedure for the rapid and reliable analysis of proteolipid protein involves the addition of sodium dodecyl sulfate to dissolve the lipid-containing protein samples or to maintain the water-soluble apoprotein in solution.
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Study of the dansylation reaction of amino acids, peptides and proteins
C. Gros,B. Labouesse +1 more
TL;DR: It is shown that a compromise for optima conditions of dansylation (pH and temperature) may be found, taking into account the rate of hydrolysis and the pK of the group to be dansyated.