Killing adherent and nonadherent cancer cells with the plasma pencil
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Citations
Treatment of gastric cancer cells with non-thermal atmospheric plasma generated in water
Temporal evaluation of the anti‐tumor efficiency of plasma‐activated media
Measurements of Plasma-Generated Hydroxyl and Hydrogen Peroxide Concentrations for Plasma Medicine Applications
Signal Amplification by Tumor Cells: Clue to the Understanding of the Antitumor Effects of Cold Atmospheric Plasma and Plasma-Activated Medium
Treatment of gastric cancer cells with nonthermal atmospheric plasma generated in water.
References
The emerging role of reactive oxygen and nitrogen species in redox biology and some implications for plasma applications to medicine and biology
Plasmas for medicine
On atmospheric-pressure non-equilibrium plasma jets and plasma bullets
Room-temperature atmospheric pressure plasma plume for biomedical applications
Arc-Free Atmospheric Pressure Cold Plasma Jets: A Review
Related Papers (5)
The emerging role of reactive oxygen and nitrogen species in redox biology and some implications for plasma applications to medicine and biology
Frequently Asked Questions (18)
Q2. What is the length of the plume?
The length of the plume depends on the gas flow rate, the magnitude of the applied voltage pulses, their widths, and the frequency.
Q3. What is the role of caspase-3 in cell death?
Caspase-3 is a protein involved in the apoptotic pathway that interacts with caspase-8 and caspase-9 through a sequential process that activates cell apoptosis.
Q4. Why are plasma bullets used in biological applications?
14Because plasma bullets are launched in air, they produce very interesting reactive chemistry that can be exploited in biological and medical applications.
Q5. What are the common reactive chemistry in the plasma pencil?
Reactive oxygen species, such as O, OH, O2 , and reactive nitrogen species, such as NO, NO2, which are known to have biological implications, are abundantly generated by the plasma bullets.
Q6. What is the role of caspases in cancer cell death?
In general, cancer cells have much higher metabolic rate than healthy cells and therefore exhibit higher levels of intracellular concentrations of ROS and RNS.
Q7. What is the effect of LTP on cells?
6,7 Keidar and coworkers reported that LTP causes an increase in the expression of the oxidative stress reporter cH2A.X (pSer 139) and a decrease in DNA replication in the S-phase of the cell cycle.
Q8. How long can a plasma plume reach?
Plasma plumes reaching lengths up to 5 cm can be launched through the hole of the outer electrode and in the surrounding room air.
Q9. How long did the cells survive in the humidified atmosphere?
They were grown in complete growth media in a vented tissue culture flask and were incubated at 37 C in a humidified atmosphere containing 5% CO2.
Q10. How many cells survived after plasma treatment?
For 5 min plasma treatment, only about 25% of cells remained viable at 24 h postplasma exposure, indicating that the higher the dose of the plasma the lower the number of the cells that survived.
Q11. How long did the cells survive after plasma treatment?
18 The results from this study indicate that there is a dose dependent response in the induction of cell death of leukemia cells and single doses of plasma treatment continue killing cells up to 2.5 days post treatment.
Q12. How long does plasma kill leukemia cells?
Initial experiments revealed that plasma kills the leukemia cells and that the effects of a single dose of plasma continue for up to few days.
Q13. What is the name of the cell line used in this study?
In this study, T-cell line (ATCC No. CCL-119; aka CCRF-CEM) originally isolated from the blood of a patient with acute lymphoblastic leukemia was used.
Q14. What is the role of caspases in cancer cell growth?
Laroussi and co-workers studied caspase-3 activation in the case of squamous cell carcinoma and found higher level of caspase-3 activation in cells treated by LTP than in control samples (untreated cells), indicating that LTP induces apoptosis in these cells.9 Ishaq et al. suggested that because tumor cells are defective in several regulatory signaling pathways they exhibit metabolic imbalance, which leads to a lack of cell growth regulation.
Q15. What is the process of releasing reactive species from the plasma?
the reactive species generated by the plasma in the gaseous state have to go through a gas–liquid interface and then diffuse into the bulk of the liquid.
Q16. How long did plasma exposure cause cell death?
starting with 24 h post-treatment, a slow but steady trend of cell death for plasma exposure times longer than 5 min was observed.
Q17. How long did the cells survive after plasma exposure?
The results revealed that the cell viability did not differ dramatically at time 0 h postplasma treatment even at the highest dose of treatment which was a plasma exposure of 10 min.
Q18. how many cells were killed after plasma pencil treatment?
The counts immediately after plasma pencil treatment at time 0 h029401-5 Laroussi, Mohades, and Barekzi: Killing adherent and nonadherent cancer cells 029401-5Biointerphases, Vol. 10, No. 2, June 2015reveal no dead cells, suggesting that there were no immediate physical effects.