Label-free photoacoustic imaging of the cardio-cerebrovascular development in the embryonic zebrafish.
TLDR
This paper applies optical resolution photoacoustic microscopy (ORPAM) to image the whole-body vasculature of the embryonic zebrafish with a special focus on the development of the cardio-cerebrovascular system, and suggests that ORPAM has the potential to investigate the cardiovascular and cerebrov vascular diseases of human in the future.Abstract:
Zebrafish play an important role in biology, pharmacology, toxicology, and medicine. The cardio-cerebrovascular development of zebrafish is particularly critical to understand both brain disorders and cardiovascular diseases in human. In this paper, we applied optical resolution photoacoustic microscopy (ORPAM) to image the whole-body vasculature of the embryonic zebrafish with a special focus on the development of the cardio-cerebrovascular system. Using the intrinsic optical absorption contrast of the embryo, we successfully visualized the formation of the cardio-cerebrovascular network in high-resolution using a 10 × objective, and monitored the whole-body vascular development using a 4 × objective. In addition, we evaluated the impact of the eggshell and pigment inhibitor on the image quality. Our results suggest that ORPAM is capable of studying the cardio-cerebrovascular development of zebrafish in the embryonic stage, and thus has the potential to investigate the cardiovascular and cerebrovascular diseases of human in the future.read more
Citations
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Cardiovascular optoacoustics: From mice to men - A review.
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Photoacoustic F-Mode imaging for scale specific contrast in biological systems
Michael J. Moore,Michael J. Moore,Eno Hysi,Eno Hysi,Muhannad N. Fadhel,Muhannad N. Fadhel,Suzan El-Rass,Suzan El-Rass,Yongliang Xiao,Yongliang Xiao,Xiao-Yan Wen,Michael C. Kolios,Michael C. Kolios +12 more
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