Journal ArticleDOI
Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens.
Sheila McCormick,Jeanne G. Niedermeyer,Joyce E. Fry,Arlene Barnason,Robert B. Horsch,Robert T. Fraley +5 more
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TLDR
The leaf disc transformation/regeneration system was modified for tomato and evidence for both single and multi-copy insertions of the T-DNA is found, and inheritance of theT-DNA insert in the expected Mendelian ratios is demonstrated.Abstract:
The leaf disc transformation/regeneration system was modified for tomato (L. esculentum). Both leaf explants and cotyledon/hypocotyl sections can be used to regenerate transformed plants. We have obtained over 300 transgenic plants from eight tomato cultivars. We have evidence for both single and multi-copy insertions of the T-DNA, and have demonstrated inheritance of the T-DNA insert in the expected Mendelian ratios. Several heterologous promoters function in tomato. A reduced efficiency of transformation was observed with binary T-DNA vectors as compared to co-integrate T-DNA vectors. The ease of the leaf disc method makes tomato a premier experimental organism for plant biotechnology.read more
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Stable Transformation of Soybean (Glycine Max) by Particle Acceleration
TL;DR: Experiments using this protocol have resulted in mature RO and R1 plants expressing the introduced gene(s), regarded as a major step in the practical application of genetic engineering to the useful modification of food-crop species.
Journal ArticleDOI
Regulation of the Amount of Starch in Plant Tissues by ADP Glucose Pyrophosphorylase
TL;DR: Plant and bacterial systems for starch and glycogen biosynthesis are similar and distinct from yeast and mammalian systems, wherein glycogen synthase has been demonstrated to be the rate-limiting regulatory step.
Journal ArticleDOI
An Ethylene-Inducible Component of Signal Transduction Encoded by Never-ripe
TL;DR: The ripening-impaired tomato mutant Never-ripe (Nr) is insensitive to the plant hormone ethylene because the gene that cosegregates with the Nr locus encodes a protein with homology to the Arabidopsis ethylene receptor ETR1 but is lacking the response regulator domain.
Journal ArticleDOI
Somatic embryogenesis from cultured leaf segments of Zea mays.
TL;DR: Basal leaf segments of 3 to 4 week old maize seedlings plated on SH medium with 30 μM dicamba produced embryogenic callus and/or somatic embryos that germinated and the resulting seedlings could be established in culture tubes.
Journal ArticleDOI
An alternative pathway to β-carotene formation in plant chromoplasts discovered by map-based cloning of Beta and old-gold color mutations in tomato
TL;DR: The results prove that beta-carotene is synthesized de novo during tomato fruit development by the B lycopene cyclase, confirming that developmentally regulated transcription is the major mechanism that governs Lycopene accumulation in ripening fruits.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
A simple and general method for transferring genes into plants
Robert B. Horsch,J. E. Fry,Nancy L. Hoffmann,Marco Wallroth,David Alan Eichholtz,Stephen G. Rogers,Robert T. Fraley +6 more
TL;DR: This method for producing transformed plants combines gene transfer, plant regeneration, and effective selection for transformants into a single process and should be applicable to plant species that can be infected by Agrobacterium and regenerated from leaf explants.
Journal ArticleDOI
Inheritance of functional foreign genes in plants.
Robert B. Horsch,Robert T. Fraley,Stephen G. Rogers,Patricia R. Sanders,Alan M Lloyd,Nancy L. Hoffmann +5 more
TL;DR: The presence of the chimeric gene in regenerated plants was demonstrated by Southern hybridization analysis, and its expression in plant tissues was confirmed by the ability of leaf segments to form callus on media containing kanamycin at concentrations that were normally inhibitory.
Journal ArticleDOI
High level expression of introduced chimaeric genes in regenerated transformed plants
TL;DR: In the most actively expressing transformants the amount of mRNA from the introduced chimaeric genes was half that of the endogenous wild‐type gene, and in general transcriptional fusions in which a linker sequence interrupted the 5′‐untranslated region gave rise to less chimaic mRNA accumulation than a translational fusion.
Journal ArticleDOI
A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts
TL;DR: With this method 1 ng of active enzyme can easily be detected and both prokaryotic and eukaryotic cell extracts can be examined, and changes in the size of enzymatically active proteins can be determined.
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