Journal ArticleDOI
Lipid analysis of Giardia lamblia and its culture medium
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Results indicate that the presence of bile extract may possibly accelerate consumption by G. lamblia of lipid in the environment.Abstract:
The neutral lipid and phospholipid composition of Giardia lamblia and its culture medium were analysed by a thin-layer chromatography flame-ionized detection system. Both lipid compositions of the parasite differed from that of the culture medium. Sterol was found to be the major neutral lipid, and phosphatidylcholine, phosphatidylethanolamine and sphingomyerin were also present. Fatty acid composition of G. lamblia and its culture medium were also analysed by gas liquid chromatography. Oleic and palmitic acid were the major fatty acids in the total lipid of the organism. The influence of porcine and bovine bile extracts on the lipid composition of G. lamblia was studied. The addition of bile to the medium caused no change in lipid composition of the parasite. Lipid composition of the culture medium was studied both before and following growth of the parasite, and it became evident that consumption of phosphatidylcholine occurred in the growth medium supplemented with bile extract. These results indicate that the presence of bile extract may possibly accelerate consumption by G. lamblia of lipid in the environment.read more
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References
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Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba
Journal ArticleDOI
Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile
TL;DR: Le milieu de Diamond TYI-S-33, utilise pour the culture axenique d'Entamoeba histolytica permet, apres addition de bile de bœuf, la culture axnique de G. lamblia.
Journal ArticleDOI
Relation of giardiasis to abnormal intestinal structure and function in gastrointestinal immunodeficiency syndromes.
Marvin E. Ament,Cyrus E. Rubin +1 more
TL;DR: After eradication of Giardia lamblia in 7 patients, the abnormalities in villus structure returned towards normal in all and the variations in the severity of the small intestinal abnormalities within individual patients meant multiple small bowel biopsies were needed to evaluate the effects of treatment on mucosal morphology.
Book ChapterDOI
Flame ionization detection applied to thin-layer chromatography on coated quartz rods.
TL;DR: This chapter describes the flame ionization detection (FID) applied to thin-layer chromatography (TLC) on coated quartz rods, which combines TLC with the FID, and the TLC/FID combination extends TLC sensitivity by two or more orders of magnitude into the range achieved only with gas chromatography.