Journal ArticleDOI
Liquid chromatography of saccharides
Reads0
Chats0
TLDR
The analysis of saccharides by liquid chromatography on an automated instrument is described in this paper, where conditions for the resolution and quantification of fructose, glucose, sucrose, melibiose, raffinose, betaine and three kestose isomers as well as starch hydrolysates are given.About:
This article is published in Journal of Chromatography A.The article was published on 1975-02-19. It has received 267 citations till now. The article focuses on the topics: Hydrophilic interaction chromatography & Melibiose.read more
Citations
More filters
Journal ArticleDOI
Probing the interaction mode in hydrophilic interaction chromatography
TL;DR: Principal component analysis of the data showed that partitioning was a dominating mechanism for uncharged solutes in HILIC, and correlations between functional groups and interactions were observed, which confirms that the HilIC retention mechanism is partly contributed by adsorption mechanisms involving electrostatic interaction and multipoint hydrogen bonding.
Journal ArticleDOI
Understanding and manipulating the separation in hydrophilic interaction liquid chromatography.
TL;DR: Progress in the understanding of the HILIC technique is covered, principally over the last ten years, including the classification of columns, the factors that control retention and selectivity, and attempts to model the separation process and its kinetics.
Journal ArticleDOI
Recent advances in peptide separation by multidimensional liquid chromatography for proteome analysis.
TL;DR: This review attempts to provide a historical perspective to separations in proteomics as well as indicate the principles of their operation and rationales for their implementation and provides a guide on what are the possibilities for combining different separation in order to increase peak capacity and proteome coverage.
Journal ArticleDOI
Recent advances and trends in the liquid-chromatography–mass spectrometry analysis of flavonoids
TL;DR: In this paper, an overview of the latest developments and their application in flavonoid analysis since 2009 is provided, focusing on methodologies which provide improved LC separation of flavonoids in terms of speed and/or resolution, including ultra high pressure LC (UHPLC), monolithic and superficially porous phases, high temperature LC (HTLC) and comprehensive two-dimensional LC (LC×LC).
Journal ArticleDOI
Efficient separation of oxidized cello-oligosaccharides generated by cellulose degrading lytic polysaccharide monooxygenases.
Bjørge Westereng,Jane Wittrup Agger,Svein Jarle Horn,Gustav Vaaje-Kolstad,Finn Lillelund Aachmann,Yngve Stenstrøm,Vincent G. H. Eijsink +6 more
TL;DR: An evaluation of HPLC-based analytical tools for the simultaneous analysis of native and oxidized cello-oligosaccharides, which are products of enzymatic cellulose degradation, found HPAEC with pulsed amperometric detection (PAD) was superior for analysis of oxidized oligosac charides, combining the best separation with superior sensitivity.
References
More filters
Related Papers (5)
Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds
Retention behavior of small polar compounds on polar stationary phases in hydrophilic interaction chromatography.
Yong Guo,Sheetal Gaiki +1 more