Journal ArticleDOI
Local Membrane Mechanics of Pore-Spanning Bilayers
Ingo Mey,Milena Stephan,Eva K. Schmitt,Martin Michael Müller,Martine Ben Amar,Claudia Steinem,Andreas Janshoff +6 more
TLDR
The mechanical behavior of lipid bilayers spanning the pores of highly ordered porous silicon substrates was scrutinized by local indentation experiments as a function of surface functionalization, lipid composition, solvent content, indentation velocity, and pore radius.Abstract:
The mechanical behavior of lipid bilayers spanning the pores of highly ordered porous silicon substrates was scrutinized by local indentation experiments as a function of surface functionalization, lipid composition, solvent content, indentation velocity, and pore radius. Solvent-containing nano black lipid membranes (nano-BLMs) as well as solvent-free pore-spanning bilayers were imaged by fluorescence and atomic force microscopy prior to force curve acquisition, which allows distinguishing between membrane-covered and uncovered pores. Force indentation curves on pore-spanning bilayers attached to functionalized hydrophobic porous silicon substrates reveal a predominately linear response that is mainly attributed to prestress in the membranes. This is in agreement with the observation that indentation leads to membrane lysis well below 5% area dilatation. However, membrane bending and lateral tension dominate over prestress and stretching if solvent-free supported membranes obtained from spreading giant liposomes on hydrophilic porous silicon are indented. An elastic regime diagram is presented that readily allows determining the dominant contribution to the mechanical response upon indentation as a function of load and pore radius.read more
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Bioinspired polymer vesicles and membranes for biological and medical applications
Cornelia G. Palivan,Roland Goers,Roland Goers,Adrian Najer,Xiaoyan Zhang,Anja Car,Wolfgang Meier +6 more
TL;DR: This work presents supramolecular polymer assemblies resulting from self-assembly of mostly amphiphilic copolymers either as 3D compartments either as polymersomes, PICsomes, peptosomes, or as planar membranes as candidates in applications ranging from drug delivery systems, up to artificial organelles, or active surfaces.
Journal ArticleDOI
Nanomechanics of Lipid Bilayers: Heads or Tails?
TL;DR: This work uses force spectroscopy AFM to quantitatively characterize the nanomechanical stability of supported lipid bilayers as a function of their chemical composition, and demonstrates that, upon introduction of cholesterol and ergosterol, the mechanical stability of membranes not only increases linearly in the liquid phase (DLPC) but also for phospholipids present in the gel phase (DPPC).
Journal ArticleDOI
Preparation and characterization of pore-suspending biomimetic membranes embedded with Aquaporin Z on carboxylated polyethylene glycol polymer cushion
Honglei Wang,Tai-Shung Chung,Yen Wah Tong,Wolfgang Meier,Zaichun Chen,Minghui Hong,Kandiah Jeyaseelan,Arunmozhiarasi Armugam +7 more
TL;DR: The carboxylated-PEG grafted porous alumina exhibits a promising platform for the fabrication of pore-suspending biomimetic membranes, and a compromise may be needed to optimize the DMPC-AQPz ratio.
Journal ArticleDOI
Multiplexed Parallel Single Transport Recordings on Nanopore Arrays
Alexander Kleefen,Daniel Pedone,Christian Grunwald,Ruoshan Wei,Matthias Firnkes,Gerhard Abstreiter,Ulrich Rant,Robert Tampé +7 more
TL;DR: A nanofabricated silicon chip is introduced for massively multiplexed analysis of membrane channels and transporters in suspended lipid membranes that does not require any surface modification or organic solvent.
Journal ArticleDOI
Giga-seal solvent-free bilayer lipid membranes: from single nanopores to nanopore arrays
TL;DR: Elect electrically sealing solvent-free bilayer lipid membranes spanned over arrays of cylindrical nanopores demonstrates the applicability of the platform to biophysical research of membrane proteins as well as pharmaceutical drug screening assays.
References
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Journal ArticleDOI
Effect of Chain Length and Unsaturation on Elasticity of Lipid Bilayers
TL;DR: To test this hypothesis, peak-to-peak headgroup thicknesses h(pp) of bilayers were obtained from x-ray diffraction of multibilayer arrays at controlled relative humidities and showed that poly-cis unsaturated chain bilayers are thinner and more flexible than saturated/monounsaturated chain Bilayers.
Journal ArticleDOI
Calculation of thermal noise in atomic force microscopy
Hans-Jürgen Butt,Manfred Jaschke +1 more
TL;DR: In this paper, the authors calculated the thermal noise of a cantilever with a free end by considering all possible vibration modes of the cantilevers and showed that if the end is supported by a hard surface, no thermal fluctuations of the deflection are possible.
Journal ArticleDOI
Entropy-driven tension and bending elasticity in condensed-fluid membranes.
Evan Evans,W. Rawicz +1 more
TL;DR: Sensitive micropipet methods have been used to measure the relation between tension and the projected surface area in fluid membranes of vesicles and confirm the prediction of equilibrium theory that the projected area should increase logarithmically with tension as shape fluctuations become progressively restricted.
Journal ArticleDOI
Elastic deformation and failure of lipid bilayer membranes containing cholesterol.
David Needham,R. S. Nunn +1 more
TL;DR: The results indicated that the presence of cholesterol is the single most influential factor in increasing bilayer cohesion, but only for lipids where both chains are saturated, or mono- or diunsaturated, and multiple unsaturation in both lipid chains inhibits the condensing effect of cholesterol in bilayers.