Journal ArticleDOI
Long-column meniscus depletion sedimentation equilibrium technique for the analytical ultracentrifuge.
TLDR
In this modification of the meniscus depletion technique for sedimentation equilibrium studies in the ultracentrifuge a relatively large volume of solvent is layered onto a small volume of sample solution in the capillary synthetic boundary cell.About:
This article is published in Analytical Biochemistry.The article was published on 1970-03-01. It has received 222 citations till now. The article focuses on the topics: Sedimentation equilibrium & Ultracentrifuge.read more
Citations
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Human copper-containing superoxide dismutase of high molecular weight
TL;DR: A superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1), distinct from previously known superoxide Dismutases, has been isolated from human lung tissue and has hydrophobic properties.
Journal ArticleDOI
The Effect of Plasmin on the Subunit Structure of Human Fibrin
TL;DR: The structural changes in the subunit structure of human fibrinogen during plasmin digestion have been followed sequentially by examining the degraded products electrophoretically in sodium dodecyl sulfate on polyacrylamide gels in the absence or presence of mercaptoethanol as mentioned in this paper.
Journal ArticleDOI
Purification and characterization of catalytic subunit of skeletal muscle adenosine 3':5'-monophosphate-dependent protein kinase.
TL;DR: The catalytic subunit of rabbit skeletal muscle cyclic adenosine 3':5'-monophosphate-dependent protein kinase has been isolated in pure form and sedimentation velocity determinations indicate that the subunit has an S20,w value of 3.12 which is essentially independent of protein concentration, interpreted as indicating that the catalyticSubunit dissociated from the holoenzyme exists as a monomer in solution.
Journal ArticleDOI
Purification of human C4b-binding protein and formation of its complex with vitamin K-dependent protein S
TL;DR: C4b-binding protein was purified from human plasma in high yield by a simple procedure involving barium citrate adsorption and two subsequent chromatographic steps, indicating two forms differing slightly from each other in molecular weight and net charge.
Journal ArticleDOI
Structural proteins of adenoviruses: II. Purification and characterization of the adenovirus type 2 fiber antigen☆
Ulf Pettersson,Stefan Höglund +1 more
TL;DR: Treatment of penton antigen with pyridine releases free vertex capsomers which are immunologically and morphologically intact and have the ability to induce cytopathic changes in KB-cell cultures.
References
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Journal ArticleDOI
Ultracentrifuge Studies with Rayleigh Interference Optics.I. General Application
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Sedimentation equilibria of polydisperse non-ideal solutes. VI Number-average molecular weight and molecular-weight distribution functions.
Journal ArticleDOI
A Fast Method for reaching Equilibrium in the Ultracentrifuge
TL;DR: In this article, the authors used the synthetic boundary cell and selected the starting conditions, such as speed of the ultracentrifuge, relative heights and the relative concentrations of the two layers, in accordance with a mathematical analysis of the approach to equilibrium.
Journal ArticleDOI
Rapid sedmentation equilibrium in the ultracentrifuge using a modified synthetic boundary cell with step concentrations
TL;DR: Rapid attainment of equilibrium was achieved without overspeeding the centrifuge by using a modified synthetic boundary cell to provide an initial multple step-concentration distribution, when compared to the reported over-speeding method.
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