Longitudinal assessment of diagnostic test performance over the course of acute SARS-CoV-2 infection.
Rebecca L. Smith,Laura Gibson,Pamela P. Martinez,Ruian Ke,Agha Mirza,Madison Conte,Nicholas Gallagher,Abigail Conte,Leyi Wang,Richard L. Fredrickson,Darci C Edmonson,Melinda E Baughman,Karen K Chiu,Hannah Choi,Tor W. Jensen,Kevin R Scardina,Shannon Bradley,Stacy L Gloss,Crystal Reinhart,Jagadeesh Yedetore,Alyssa N Owens,John Broach,Bruce A. Barton,Peter Lazar,Darcy Henness,Todd Young,Alastair Dunnett,Matthew L Robinson,Heba H. Mostafa,Andrew Pekosz,Yukari C. Manabe,William J Heetderks,David D. McManus,Christopher B. Brooke +33 more
TLDR
In a longitudinal study of 43 adults newly infected with SARS-CoV-2, all provided daily saliva and nasal swabs for quantitative reverse transcription polymerase chain reaction (RT-qPCR), Quidel SARS Sofia antigen fluorescent immunoassay (FIA), and live virus culture as mentioned in this paper.Abstract:
BACKGROUND Serial screening is critical for restricting spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by facilitating timely identification of infected individuals to interrupt transmission. Variation in sensitivity of different diagnostic tests at different stages of infection has not been well documented. METHODS In a longitudinal study of 43 adults newly infected with SARS-CoV-2, all provided daily saliva and nasal swabs for quantitative reverse transcription polymerase chain reaction (RT-qPCR), Quidel SARS Sofia antigen fluorescent immunoassay (FIA), and live virus culture. RESULTS Both RT-qPCR and Quidel SARS Sofia antigen FIA peaked in sensitivity during the period in which live virus was detected in nasal swabs, but sensitivity of RT-qPCR tests rose more rapidly prior to this period. We also found that serial testing multiple times per week increases the sensitivity of antigen tests. CONCLUSIONS RT-qPCR tests are more effective than antigen tests at identifying infected individuals prior to or early during the infectious period and thus for minimizing forward transmission (given timely results reporting). All tests showed >98% sensitivity for identifying infected individuals if used at least every 3 days. Daily screening using antigen tests can achieve approximately 90% sensitivity for identifying infected individuals while they are viral culture positive.read more
Citations
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Daily longitudinal sampling of SARS-CoV-2 infection reveals substantial heterogeneity in infectiousness
Ruian Ke,Pamela P. Martinez,Rebecca L. Smith,Laura Gibson,Agha Alfi Mirza,M. Conte,Nichola Gallagher,Chunmei Luo,Junko Jarrett,Ruifeng Zhou,A. Conte,Tongyu Liu,M. Farjo,Kimberly K. O. Walden,Gloria Rendon,Christopher J. Fields,Leyi Wang,Richard L. Fredrickson,Darci C Edmonson,M. Baughman,Karen Chiu,Hannah Choi,K. Scardina,S. Bradley,Stacy L Gloss,Crystal Reinhart,J. Yedetore,Jessica Quicksall,Alyssa N Owens,J. Broach,Bruce A. Barton,Peter Lazar,W.J. Heetderks,Matthew L Robinson,Heba H. Mostafa,Yukari C. Manabe,Andrew Pekosz,David D. McManus,C. Brooke +38 more
TL;DR: In this article , the dynamics of infectious virus and viral RNA shedding during acute infection through daily longitudinal sampling of 60 individuals for up to 14 days were captured by fitting mechanistic models, and they directly estimated viral expansion and clearance rates and overall infectiousness for each individual.
Journal ArticleDOI
Comparison of Home Antigen Testing With RT-PCR and Viral Culture During the Course of SARS-CoV-2 Infection.
Victoria T Chu,Noah G. Schwartz,Marisa Anne Pella Donnelly,Meagan Chuey,Raymond Soto,Anna R Yousaf,Emily N. Schmitt-Matzen,Sadia Sleweon,Jasmine Ruffin,Natalie J. Thornburg,Jennifer L Harcourt,Azaibi Tamin,Gimin Kim,Jennifer Fostel,Laura Hughes,Suxiang Tong,Ginger Stringer,Bernadette A. Albanese,Sarah E. Totten,Meghan M. Hudziec,Shannon R. Matzinger,Elizabeth A. Dietrich,Sarah W. Sheldon,Sarah S Stous,Eric McDonald,B. Austin,Mark E. Beatty,J. Erin Staples,Marie E Killerby,Christopher H. Hsu,Jacqueline E. Tate,Hannah L Kirking,Almea Matanock +32 more
TL;DR: The results of this cohort study of home antigen tests suggest that sensitivity for SARS-CoV-2 was moderate compared with RT-PCR and high compared with viral culture and should be tested again 1 to 2 days later because test sensitivity peaked several days after illness onset and improved with repeated testing.
Journal ArticleDOI
Rapid, point-of-care antigen tests for diagnosis of SARS-CoV-2 infection
TL;DR: In this article , the second update of this review was published, which was first published in 2020, and included 152 evaluations of single test applications including 100,462 unique samples (16,822 with confirmed SARS-CoV-2 infection or known absence of infection).
Journal ArticleDOI
Quantitative SARS-CoV-2 Viral-Load Curves in Paired Saliva Samples and Nasal Swabs Inform Appropriate Respiratory Sampling Site and Analytical Test Sensitivity Required for Earliest Viral Detection
TL;DR: In this article , the authors demonstrate the value of acquiring early (within hours after a negative high-sensitivity test) viral-load profiles to guide the appropriate analytical sensitivity and respiratory site for detecting earliest infections.
Posted ContentDOI
Longitudinal analysis of SARS-CoV-2 vaccine breakthrough infections reveal limited infectious virus shedding and restricted tissue distribution
Ruian Ke,Pamela P. Martinez,Rebecca L. Smith,Laura Gibson,Chad J. Achenbach,Sally M. McFall,Chao Qi,Joshua Jacob,Etienne Dembele,Camille Bundy,Lacy M Simons,Egon A. Ozer,Judd F. Hultquist,Ramon Lorenzo-Redondo,Anita Opdycke,Claudia Hawkins,Robert L. Murphy,Agha Mirza,Madison Conte,Nicholas Gallagher,Chun Huai Luo,Junko Jarrett,Abigail Conte,Ruifeng Zhou,Mireille Farjo,Gloria Rendon,Christopher J. Fields,Leyi Wang,Richard L. Fredrickson,Melinda E Baughman,Karen Chiu,Hannah Choi,Kevin R Scardina,Alyssa N Owens,John Broach,John Broach,Bruce A. Barton,Peter Lazar,Matthew L Robinson,Heba H. Mostafa,Yukari C. Manabe,Andrew Pekosz,David D. McManus,Christopher B. Brooke +43 more
TL;DR: In this article, the authors examined viral dynamics and infectious virus shedding through daily longitudinal sampling in a small cohort of adults infected with SARS-CoV-2 at varying stages of vaccination.
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