Low-power noncontact photoacoustic microscope for bioimaging applications
K. Sathiyamoorthy,K. Sathiyamoorthy,Eric M. Strohm,Eric M. Strohm,Michael C. Kolios,Michael C. Kolios +5 more
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TLDR
An inexpensive noncontact photoacoustic (PA) imaging system using a low-power continuous wave laser and a kilohertz-range microphone has been developed, enabling optical and PA imaging of the same sample.Abstract:
An inexpensive noncontact photoacoustic (PA) imaging system using a low-power continuous wave laser and a kilohertz-range microphone has been developed. The system operates in both optical and PA imaging modes and is designed to be compatible with conventional optical microscopes. Aqueous coupling fluids are not required for the detection of the PA signals; air is used as the coupling medium. The main component of the PA system is a custom designed PA imaging sensor that consists of an air-filled sample chamber and a resonator chamber that isolates a standard kilohertz frequency microphone from the input laser. A sample to be examined is placed on the glass substrate inside the chamber. A laser focused to a small spot by a 40 × objective onto the substrate enables generation of PA signals from the sample. Raster scanning the laser over the sample with micrometer-sized steps enables high-resolution PA images to be generated. A lateral resolution of 1.37 ?? ? m was achieved in this proof of concept study, which can be further improved using a higher numerical aperture objective. The application of the system was investigated on a red blood cell, with a noise-equivalent detection sensitivity of 43,887 hemoglobin molecules ( 72.88 × 10 ? 21 ?? mol or 72.88 zeptomol). The minimum pressure detectable limit of the system was 19.1 ?? ? Pa . This inexpensive, compact noncontact PA sensor is easily integrated with existing commercial optical microscopes, enabling optical and PA imaging of the same sample. Applications include forensic measurements, blood coagulation tests, and monitoring the penetration of drugs into human membrane.read more
Citations
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Photoacoustic imaging in the second near-infrared window: a review
TL;DR: This work highlights the importance of the second window for PA imaging and discusses the various NIR-II PA imaging systems and contrast agents with strong absorption in the Nir-II spectral region, including whole-body animal imaging and human imaging.
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Towards non-contact photoacoustic imaging [review].
TL;DR: This paper presents a brief overview of current non-contact photoacoustic detection techniques with an emphasis on all-optical detection methods and their associated physical mechanisms.
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Fiber interferometer for hybrid optical and optoacoustic intravital microscopy
Rami Shnaiderman,Georg Wissmeyer,Markus Seeger,Dominik Soliman,Héctor Estrada,Daniel Razansky,Amir Rosenthal,Vasilis Ntziachristos +7 more
TL;DR: In this article, the authors describe a miniaturized optoacoustic sensor, based on a π-phase-shifted fiber Bragg grating embedded in an acoustic cavity, which can be seamlessly integrated into any conventional optical microscope.
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Dual modality reflection mode optical coherence and photoacoustic microscopy using an akinetic sensor.
Richard Haindl,Stefan Preisser,Marco Andreana,Wolfgang Rohringer,Caterina Sturtzel,Martin Distel,Zhe Chen,Elisabet Rank,Balthasar Fischer,Wolfgang Drexler,Mengyang Liu +10 more
TL;DR: The OC-PAM system was applied to image zebrafish larvae in vivo, demonstrating its capability in biomedical imaging with complementary optical scattering and absorption contrasts by revealing morphology in the fish larvae.
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TL;DR: Functional photoacoustic microscopy (fPAM) is reported, which provides multiwavelength imaging of optical absorption and permits high spatial resolution beyond this depth limit with a ratio of maximum imaging depth to depth resolution greater than 100.
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TL;DR: The recognized leader in the modern development of the field presents both theory and applications as mentioned in this paper, and provides a comprehensive survey of applications, from spectroscopy and chemistry analysis to medical applications and thermal analysis.