Journal ArticleDOI
Measurement of chemiluminescence in freshly drawn human blood. II. Clinical application of zymosan-induced chemiluminescence.
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TLDR
In a small number of patients with benign and malignant disease the CL of PC was measured both pre- and postoperatively, and operations in benign disease and palliative operations in malignancy did not influence the CL activity.Abstract:
Summary. The aim of the present study was to investigate chemiluminescence (CL) of stimulated peripheral phagocytic cells (PC, i.e. granulocytes and monocytes) in patients with malignant disease at various stages. As a first step the zymosan-induced and luminol-amplified CL was determined in diluted whole blood samples from healthy volunteers. A characteristic daytime dependence of the CL activity was observed in six volunteers which had to be taken into account for blood sampling. The detectable CL was demonstrated to depend on the number of erythrocytes in the assay, but correction for this is not neccessary for clinical investigation. The specific CL activity (activity related to 10 3 PC, was significantly but identically increased both in 1) patients with acute inflammatory disease and 2) in patients with carcinoma. The total CL activity (activity/gl whole blood), however, was significantly increased in patients with acute inflammation as compared to the tumour~ group. This greatly reflects the leucocytosis of patients with acute inflammation. In a small number of patients with benign and malignant disease the CL of PC was measured both pre- and postoperatively. Operations in benign disease and palliative operations in malignancy did not influence the CL activity. In contrast, CL activity returned to normal after clinical cure by radical tumour resection.read more
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Antimicrobial and antioxidant activities of coumarins from the roots of Ferulago Campestris (Apiaceae)
Adriana Basile,Sergio Sorbo,Vivienne Spadaro,Maurizio Bruno,Antonella Maggio,Nicoletta Faraone,Sergio Rosselli +6 more
TL;DR: Aegelinol and agasyllin showed antibacterial activity against nine ATCC and the same clinically isolated Gram-positive and Gram-negative bacterial strains, as well as the antibacterial and antioxidant activities of the three most abundant ones.
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Antimicrobial and antioxidant activities of Feijoa sellowiana fruit
Maria Luisa Vuotto,Adriana Basile,V. Moscatiello,Pasquale De Sole,R. Castaldo-Cobianchi,Emanuela Laghi,Maria Teresa Lucia Ielpo +6 more
TL;DR: The tropical Feijoa sellowiana Berg fruit showed both antibacterial and antioxidant properties and therefore its extract might be used as a new multifaceted drug.
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Antibacterial and antioxidant activities in Sideritis italica (Miller) Greuter et Burdet essential oils.
Adriana Basile,Felice Senatore,Rosalba Gargano,Sergio Sorbo,Marisa Del Pezzo,Alfredo Lavitola,Alberto Ritieni,Maurizio Bruno,Daniela Spatuzzi,Daniela Rigano,Maria Luisa Vuotto +10 more
TL;DR: The finding of antibacterial and antioxidant activities in the essential oils from Sideritis italica flowerheads and leaves validated the use of the plant for medical purposes.
Journal ArticleDOI
Immunopharmacological properties of flavonoids.
Mtl Ielpo,Adriana Basile,R Miranda,V. Moscatiello,C Nappo,S Sorbo,E Laghi,M.M Ricciardi,L Ricciardi,Maria Luisa Vuotto +9 more
TL;DR: Evaluating the effects of acetonic extracts from Lunularia cruciata gametophytes and Feijoa sellowiana fruits on human phagocyte functions demonstrates that both the raw extracts and flavonoids significantly inhibit CL emission by leukocytes, especially when these cells are activated by PMA.
Journal ArticleDOI
In vitro determination of phagocyte activity by luminol- and lucigenin-amplified chemiluminescence.
TL;DR: Lucigenin-amplified CL offers great advantages to study cell activating or inhibiting properties of drugs and kinetics in vitro because of the biological relevance of O-.2 determination, its sensitivity, reproducibility and ease in handling.
References
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Journal ArticleDOI
Oxygen-dependent microbial killing by phagocytes (second of two parts).
TL;DR: Since Metschnikoff's discovery, hundreds of scientists studying dozens of species have reported thousands of studies on these cells, perhaps the most widely recognized of which are those of the eminent English scientists.
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Active Oxygen Species and the Functions of Phagocytic Leukocytes
Journal ArticleDOI
Evidence for the generation of an electronic excitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity
TL;DR: It is proposed that the CL of PMN reflects the generation of singlet oxygen, 1 0 2 , which acts per , se , as the bactericidal and/or CL species.
Journal Article
Polymorphonuclear leukocyte-mediated, antibody-dependent, cellular cytotoxicity against tumor cells: dependence on oxygen and the respiratory burst.
Dean G. Hafeman,Zoltan J. Lucas +1 more
TL;DR: Experiments were done to determine whether the respiratory burst of superoxide anion (O2-) production in polymorphonuclear leukocytes (PMN) is triggered during antibody-dependent killing of tumor cells and whether O2- production is essential for cytotoxicity.
Journal Article
Studies on the mechanism of antibody-dependent polymorphonuclear leukocyte-mediated cytotoxicity.
TL;DR: It is suggested that antibody-dependent PMN-mediated tumor cell cytotoxicity requires specific antibody, viable PMN, a burst of PMN oxidative metabolism, glycolysis, divalent cations, and microtubular function.