Medium Promoting Sporulation of Bacillus larvae and Metabolism of Medium Components.
D. W. Dingman,Donald P. Stahly +1 more
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A new medium, designated TMYGP broth, was developed that allowed the honeybee pathogen Bacillus larvae NRRL B-3650 to produce up to 5 x 10 spores per ml of culture (microscopic count).Abstract:
A new medium, designated TMYGP broth, was developed that allowed the honeybee pathogen Bacillus larvae NRRL B-3650 to produce up to 5 × 108 spores per ml of culture (microscopic count). This species normally sporulates poorly, if at all, in artificial broth media. An aeration rate lower than that normally used to cultivate other Bacillus species was required for sporulation. During the exponential growth phase, acids were produced by catabolism of yeast extract components, causing a decrease in pH of the medium. Thereafter, the pH began to increase, probably because of derepression of the citric acid cycle and consumption of the acids. Only after this time did usage of glucose from the medium occur. Thus, glucose usage seems to be regulated by catabolite repression. The presence of glucose was needed for one or more of the later events of sporulation. Of many substances tested, only gluconic acid and glucosamine partially substituted for glucose as a requirement for sporulation. Pyruvate was also required for good sporulation. It was metabolized during the late-exponential phase of growth.read more
Citations
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Bacillus thuringiensis and related insect pathogens.
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American foulbrood: a review of its biology, diagnosis and control
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Standard methods for American foulbrood research.
Dirk C. de Graaf,Adriana Mónica Alippi,Karina Antúnez,Katherine A Aronstein,Giles E. Budge,Lina De Smet,Douglas W. Dingman,Jay D. Evans,Leonard J. Foster,Anne Fünfhaus,K Daniel Murray,Bach Kim Nguyen,Lena Poppinga,Marla Spivak,Selwyn Wilkins,Elke Genersch,Investigaciones Biológicas,Clemente Estable,Barc-E Bldg,Gembloux Agro-Bio Tech +19 more
TL;DR: Techniques and protocols for American foulbrood research are provided, mostly in a recipe-like format that permits easy implementation in the laboratory and other technical information including biosafety measures to guarantee the safe handling of this pathogen is covered.
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Characterization of microorganisms in Argentinean honeys from different sources.
Miriam O. Iurlina,R. Fritz +1 more
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Properties of different lactic acid bacteria isolated from Apis mellifera L. bee-gut
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References
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Journal ArticleDOI
Analysis of Sporulation Mutants II. Mutants Blocked in the Citric Acid Cycle
Peter Fortnagel,Ernst Freese +1 more
TL;DR: The intact Krebs cycle is therefore not required for vegetative growth of aerobic Bacillis subtilis, but it is indispensable for sporulation.
Book ChapterDOI
The Biochemistry of the Bacterial Endospore
TL;DR: This chapter focuses on the present chemical knowledge of the mature spore along with theBiochemistry of its formation, and presents definitions and cytological changes during sporogenesis that help in assisting the understanding of the biochemistry of the spore.
Journal ArticleDOI
Bacteria as insect pathogens.
TL;DR: The objective was to establish an experimental procedure and show direct AFM measurements that unequivocally can be assigned ascanonical for the response of the immune system to E.coli.
Journal ArticleDOI
The Mechanism of Colony Resistance to American Foulbrood
A. W. Woodrow,E. C. Holst +1 more