Journal ArticleDOI
Micellar capillary electrophoresis of the ecdysteroids
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TLDR
The micellar capillary electrophoresis technique was found to be suitable for the analysis of purified ecdysteroid-rich extracts of plants and the eggs of the desert locust and the elution order of the analytes was similar to that obtained using reversed-phase HPLC.Abstract:
The technique of micellar capillary electrophoresis has been applied to the separation of ecdysteroids (polyhydroxylated insect hormones) both as pure standards and in the extracts of plants and insect eggs. Succesful separations of a range of closely related ecdysteroids were obtained, however, the quality of the result was found to be critically dependent on the solvent in which the sample was applied and the degree of purity of the extracts. The technique was found to be suitable for the analysis of purified ecdysteroid-rich extracts of plants (Silene nutans, S. otites) and the eggs of the desert locust (Schistocerca gregaria). The elution order of the analytes was similar to that obtained using reversed-phase HPLC.read more
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Book ChapterDOI
Ecdysteroid Chemistry and Biochemistry
TL;DR: In this article, the authors focus on the techniques used for ecdysteroid analysis and the biosynthesis and metabolism of these hormones, and the greatest diversity has been found in the eggs/embryos of Orthoptera and Lepidoptera.
Journal ArticleDOI
Micelles as pseudo-stationary phases in micellar electrokinetic chromatography.
TL;DR: This review article describes some general comments on micellar electrokinetic chromatography from the viewpoint of pseudo-stationary phases and presents a compiled list of surfactants used for MEKC, prepared from published papers.
Journal ArticleDOI
Distribution of phytoecdysteroids in the Caryophyllaceae.
L. N. Zibareva,Vladimir A. Volodin,Zyadilla Saatov,Tamara Savchenko,Pensri Whiting,René Lafont,Laurence Dinan +6 more
TL;DR: It was found that phytoecdysteroids were present predominantly in the Genera Lychnis, Petrocoptis, Sagina and Silene, and close association of ecdysteroid occurrence with certain groups of this tribe was revealed.
Journal ArticleDOI
Separation of estrogens by micellar electrokinetic chromatography
TL;DR: Capillary electrophoresis of the sex hormone estrogens using different buffer components was investigated and the addition of modifiers such as organic solvents or cyclodextrins improved resolutions significantly.
References
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BookDOI
Chromatography and isolation of insect hormones and pheromones
TL;DR: The Precocene Antijuvenile Hormones (Allatotoxins) and the Regulation of the Corpora Allata by the Brain in the Adult Female American Cockroach, Periplaneta americana are studied.