Microscopy with self-reconstructing beams
TLDR
In this paper, a self-reconstructing Bessel beam was used to reduce scattering artifacts and increase image quality and penetration depth in three-dimensional inhomogeneous opaque media.Abstract:
A prototype microscope built with self-reconstructing Bessel beams is shown to be able to reduce scattering artifacts as well as increase image quality and penetration depth in three-dimensional inhomogeneous opaque media.read more
Citations
More filters
Journal ArticleDOI
Controlling waves in space and time for imaging and focusing in complex media
TL;DR: In this article, the authors used strongly scattering materials to focus, shape and compress waves by controlling the many degrees of freedom in the incident waves in complex media such as white paint and biological tissue.
Journal ArticleDOI
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
Thomas A. Planchon,Liang Gao,Daniel E. Milkie,Michael W. Davidson,James A. Galbraith,Catherine G. Galbraith,Eric Betzig +6 more
TL;DR: Scanned Bessel beams are used in conjunction with structured illumination and/or two-photon excitation to create thinner light sheets better suited to three-dimensional (3D) subcellular imaging.
Journal ArticleDOI
Light-sheet microscopy using an Airy beam
Tom Vettenburg,Heather Isla Campbell Dalgarno,Jonathan Nylk,Clara Coll-Lladó,David E. K. Ferrier,Tomáš Čižmár,Frank J. Gunn-Moore,Kishan Dholakia +7 more
TL;DR: It is shown that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV, and its characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.
Journal ArticleDOI
Optical pulling force
TL;DR: Theoretical analysis suggests that there exists an optical attractive force capable of "pulling" microparticles towards a light source as mentioned in this paper, which is generated by using interference to optimize the scattering of light in the forwards direction.
Journal ArticleDOI
A guide to light-sheet fluorescence microscopy for multiscale imaging
TL;DR: This work elucidate the key developments and define a simple set of underlying principles governing LSFM, which aim to clarify the decisions to be made for those who wish to develop and use bespoke light-sheet systems and to assist in identifying the best approaches to apply this powerful technique to myriad biological questions.
References
More filters
Journal ArticleDOI
Diffraction-free beams.
TL;DR: The first experimental investigation of nondiffracting beams, with beam spots as small as a few wavelengths, can exist and propagate in free space, is reported.
Journal ArticleDOI
Optical sectioning deep inside live embryos by selective plane illumination microscopy
TL;DR: In this article, a selective plane illumination microscopy (SPIM) was developed to generate multidimensional images of samples up to a few millimeters in size, which can be applied to visualize the embryogenesis of the relatively opaque Drosophila melanogaster in vivo.
Journal ArticleDOI
Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy.
TL;DR: This work developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development to derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event.
Journal ArticleDOI
Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain.
Hans-Ulrich Dodt,Ulrich Leischner,Anja Schierloh,Nina Jährling,Nina Jährling,Christoph P. Mauch,Katrin Deininger,Jan M. Deussing,Matthias Eder,Walter Zieglgänsberger,Klaus Becker,Klaus Becker +11 more
TL;DR: This new technique allows optical sectioning of fixed mouse brains with cellular resolution and can be used to detect single GFP-labeled neurons in excised mouse hippocampi and is ideally suited for high-throughput phenotype screening of transgenic mice and thus will benefit the investigation of disease models.