MiR-27a-3p enhances the cisplatin sensitivity in hepatocellular carcinoma cells through inhibiting PI3K/Akt pathway.
Ying Yang,Zhifang Yang,Rui-Li Zhang,Chunli Jia,Rui Mao,Sha Ya,Yue-fen Zhang,Ge Wu,Yan Na Sun,Xiao Yan Jia,Ainiwaer Aimudula,Hua Zhang,Yong-xing Bao +12 more
TLDR
In this paper, the miR27a-3p expression levels in 372 tumor tissues and 49 adjacent tissues in HCC samples from TCGA database, and found that the level of miR-27a3p was associated with metastasis, Child-Pugh grade and race.Abstract:
MicroRNAs (miRNAs) play an important role in drug-resistance, and it's reported that MiR-27a-3p regulated the sensitivity of cisplatin in breast cancer, lung cancer and ovarian cancer. However, the relationship between miR-27a-3p and chemosensitivity of cisplatin in HCC was unclear, especially the underlying mechanism was unknown. In present study, we analyzed miR-27a-3p expression levels in 372 tumor tissues and 49 adjacent tissues in HCC samples from TCGA database, and found that the miR-27a-3p was downregulated in HCC tissues. The level of miR-27a-3p was associated with metastasis, Child-Pugh grade and race. MiR-27a-3p was regarded as a favorable prognosis indicator for HCC patients. Then, miR-27a-3p was overexpressed in HepG2 cell, and was knockdown in PLC cell. Next, we conducted a series of vitro assays, including MTT, apoptosis and cell cycle assays to observe the biological changes. Further, inhibitor rate and apoptosis rate were detected with pre- and post-cisplatin treatment in HCC. The results showed that overexpression of miR-27a-3p repressed the cell viability, promoted apoptosis and increased the percentage of cells in phase G0/G1 phase. Importantly, overexpression of miR-27a-3p significantly increased the inhibitor rate and apoptosis rate with cisplatin intervention. Besides, we found that miR-27a-3p added cisplatin sensitivity potentially through regulating PI3K/Akt signaling pathway. Taken together, MiR-27a-3p acted as a tumor suppressor gene in HCC cells, and it could be useful for modulating cisplatin sensitivity in chemotherapy therapy.read more
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Wnt/β-Catenin Signaling as a Driver of Hepatocellular Carcinoma Progression: An Emphasis on Molecular Pathways.
TL;DR: In this article, the role of Wnt signaling in hepatocellular carcinoma (HCC) and its association with progression and therapy response based on pre-clinical and clinical evidence is discussed.
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Non-coding RNA in cancer drug resistance: Underlying mechanisms and clinical applications
Xu Zhou,Xiang Ao,Zhaojun Jia,Yiwen Li,Shouxiang Kuang,C. Du,Jin-yu Zhang,Jianxun Wang,Ying Liu +8 more
TL;DR: The recent findings on the emerging role and underlying mechanisms of ncRNAs involved in cancer drug resistance are summarized and their clinical applications as biomarkers and therapeutic targets in cancer treatment are focused on.
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Biological function and therapeutic perspective of targeting PI3K/Akt signaling in hepatocellular carcinoma: promises and challenges.
Mahshid Deldar Abad Paskeh,Fatemeh Ghadyani,M. Hashemi,Ali Reza Abbaspour,Amirhossein Zabolian,Salar Javanshir,Mehrnaz Razzazan,Sepideh Mirzaei,Maliheh Entezari,Mohammad Ali Sheikh Beig Goharrizi,Shokooh Salimimoghadam,Amir Reza Aref,Alireza Kalbasi,Romina Rajabi,Mohsen Rashidi,Afshin Taheriazam,Gautam Sethi +16 more
TL;DR: In this article , the role of PI3K/Akt signaling in hepatocellular carcinoma (HCC) progression was investigated, and it was shown that PI3k/akt signaling promotes glucose uptake, favors glycolysis and increases tumor cell proliferation.
Journal ArticleDOI
MiR-27a-3p binds to TET1 mediated DNA demethylation of ADCY6 regulates breast cancer progression via epithelial-mesenchymal transition
TL;DR: It is found that ADCY 6 is expressed at low levels in breast cancer and leads to increases in the proliferation, invasion and migration of breast cancer cells, and bioinformatics analysis showed that TET1 is regulated by miR-27a-3p and regulates the methylation of ADCY6 to affect the EMT process of breastcancer cells, thereby affecting the malignant biological behaviour of breast cancers.
Journal ArticleDOI
miR‑27a‑3p upregulation by p65 facilitates cervical tumorigenesis by increasing TAB3 expression and is involved in the positive feedback loop of NF‑κB signaling
TL;DR: In this article , an altered microRNA (miRNA/miR)-27a-3p expression has been identified in cervical cancer, while the exact regulatory mechanisms responsible for the dysregulation of miR-27a3p remain to be fully elucidated.
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