Mitochondrial aggregation patterns and activity in human oocytes and preimplantation embryos
Martin Wilding,Brian Dale,Marcella Marino,Loredana Di Matteo,Carlo Alviggi,Maria Laura Pisaturo,Luisa Lombardi,Giuseppe De Placido +7 more
TLDR
The data suggest that the efficiency of mitochondrial respiration in oocytes and preimplantation embryos is closely correlated with the programmed rate of embryo development, and suggest that maternal age further influences this factor.Abstract:
Mitochondria play a vital role in the metabolism of energy-containing compounds in the oocyte cytoplasm to provide adenosine trisphosphate for fertilization and preimplantation embryo development. In this study, ratiometric confocal microscopy with the mitochondrion-specific membrane potential-sensitive fluorescence dye JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide) was used to measure the activity of mitochondria in human oocytes and developing preimplantation embryos. Mitochondria in oocytes and embryos were characterized by distinct localized aggregation patterns. These patterns however did not determine localized regions of heterogeneity in mitochondrial activity. Mitochondrial activity was analysed during oocyte maturation and after fertilization. The activity of mitochondria in fresh metaphase II oocytes was negatively correlated with maternal age. This trend continued when the activity of developing embryos was analysed. Mitochondrial activity was strongly correlated with the rate of embryo development on day 3 after fertilization, but not on day 2. Partial regression analysis showed that the rate of cleavage of preimplantation embryos was more highly correlated with embryo mitochondrial activity than maternal age. These data suggest that the efficiency of mitochondrial respiration in oocytes and preimplantation embryos is closely correlated with the programmed rate of embryo development, and suggest that maternal age further influences this factor. The loss of mitochondrial activity in oocytes obtained from ageing couples may therefore contribute to lower embryo development and pregnancy rates observed during cycles of IVF.read more
Citations
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Energy Metabolic Phenotype of the Cardiomyocyte During Development, Differentiation, and Postnatal Maturation
TL;DR: If a hypertrophic stress is placed on the adult heart, cardiac energy metabolism switches to a more fetal phenotype, which includes an increase in glycolysis and decrease in mitochondrial fatty acid β-oxidation.
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Mitochondrial gene replacement in primate offspring and embryonic stem cells
Masahito Tachibana,Michelle Sparman,Hathaitip Sritanaudomchai,Hong Ma,Lisa Clepper,Joy Woodward,Ying Li,Cathy Ramsey,Olena Kolotushkina,Shoukhrat Mitalipov,Shoukhrat Mitalipov +10 more
TL;DR: It is demonstrated here that the mitochondrial genome can be efficiently replaced in mature non-human primate oocytes (Macaca mulatta) by spindle–chromosomal complex transfer from one egg to an enucleated, mitochondrial-replete egg.
Journal ArticleDOI
Mitochondrial function in the human oocyte and embryo and their role in developmental competence.
TL;DR: The notion of functional compartmentalization of mitochondria is presented as a means by which ATP may be differentially supplied and localized within the cytoplasm by virtue of stage-specific changes in mitochondrial density and potential.
Book ChapterDOI
The role of mitochondrial function in the oocyte and embryo.
TL;DR: A dual role for mitochondria (to maintain life or to commit to cell death) may well represent a quality control system in the early embryo that will determine whether the embryo proceeds further into development or is quickly eliminated.
Journal ArticleDOI
Oocyte aging: cellular and molecular changes, developmental potential and reversal possibility
TL;DR: Oocyte aging can be delayed or reversed by various treatments to increase success rates and produce increased numbers of healthy embryos, preventing failures or abnormalities that are frequently associated with ART using aged oocytes.
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