Molecular characterization of extended-spectrum β-lactamases in clinical Escherichia coli and Klebsiella pneumoniae isolates from Surabaya, Indonesia
Juliëtte A. Severin,Ni Made Mertaniasih,Kuntaman Kuntaman,Endang Sri Lestari,Marijam Purwanta,Nicole Lemmens-den Toom,D. Offra Duerink,Usman Hadi,Alex van Belkum,Henri A. Verbrugh,Wil H. F. Goessens +10 more
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TLDR
Indonesia is another developing country affected by the emergence and spread of bacterial strains harbouring ESBL genes, including the CTX-M-15-producing B2-E.Abstract:
BACKGROUND: No detailed reports regarding extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are currently available from Indonesia, the fourth most populous country in the world. METHODS: A survey was carried out to investigate the molecular epidemiology and genetic characteristics of clinical ESBL-producing Escherichia coli and Klebsiella pneumoniae isolates originating from the Dr. Soetomo Academic Hospital in Surabaya, Indonesia, over a 4 month period (January to April 2005). ESBLs were characterized by isoelectric focusing and PCR assays. Clonality of the isolates was assessed by PFGE and repetitive-sequence-based PCR (rep-PCR). Phylogenetic grouping was performed among CTX-M-15-producing E. coli. RESULTS: In total, 73 consecutive non-duplicate ESBL-positive E. coli and 72 K. pneumoniae strains were isolated. The bla(CTX-M-15) gene was found to be highly prevalent (69/73 strains, 94.5%) among the 73 ESBL-positive E. coli isolates. The gene was detected in both clonal and non-clonal isolates, as defined by PFGE and rep-PCR. Sixteen CTX-M-15-positive E. coli could be assigned to a single rep-PCR type and phylogenetic group B2 and belonged to the well-known O25b-ST131 clone. Among the 72 ESBL-positive K. pneumoniae isolates, bla(CTX-M-15) was again the most prevalent ESBL (40/72, 55.6%). Several SHV-type enzymes were also frequently detected: SHV-5 (n = 28); SHV-12 (n = 13); and SHV-2 (n = 6). TEM-type ESBLs were not detected in any of the isolates. CONCLUSIONS: Indonesia is another developing country affected by the emergence and spread of bacterial strains harbouring ESBL genes, including the CTX-M-15-producing B2-E. coli O25b-ST131 clone.read more
Citations
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Escherichia coli O25b-ST131: a pandemic, multiresistant, community-associated strain
TL;DR: Phenotypic detection of the ST131 clone is not possible and DNA-based techniques, including MLST and PCR, are described, and a broad distribution has been demonstrated amongst antimicrobial-resistant E. coli.
Journal ArticleDOI
Extended-spectrum [beta]-lactamase producing Escherichia coli: changing epidemiology and clinical impact.
TL;DR: The worldwide emergence of multiresistant ESBL-producing E. coli raises key therapeutic problems; interventions addressed to their quick detection and early appropriate antibiotic treatment and prevention are urgently needed.
Journal ArticleDOI
The CTX-M Conundrum: Dissemination of Plasmids and Escherichia coli Clones
Umaer Naseer,Arnfinn Sundsfjord +1 more
TL;DR: A global epidemic of bla(CTX-M)-encoding E. coli strains and plasmids are witnessing a global epidemic, which require serious attention and efficient infection control measures.
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Carbapenem and colistin resistance in Enterobacteriaceae in Southeast Asia: Review and mapping of emerging and overlapping challenges
TL;DR: The widespread presence of carbapenem and polymyxin resistance, including their overlap in 8 nations, represents a continuing risk and increases the threat of infections resistant to both and highlights the urgent need for sufficiently resourced robust antimicrobial resistance surveillance.
Journal ArticleDOI
Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli in Riyadh: emergence of CTX-M-15-producing E. coli ST131.
Mohamed H. Al-Agamy,Mohamed H. Al-Agamy,Atef M. Shibl,Atef M. Shibl,Mohamed M. Hafez,Mohamed M. Hafez,Mohammad N. Al-Ahdal,Ziad A. Memish,Harish Khubnani +8 more
TL;DR: This study documented the high prevalence of ESBLs in E. coli isolates, with CTX-M-15 as the predominant ESBL gene, and the detection of the ST131 clone in Saudi E.coli isolates.
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