Journal ArticleDOI
Molecular Characterization of Pseudomonas putida Group Isolates Carrying blaVIM-2 Disseminated in a University Hospital in Korea.
Jun Sung Hong,Eun Jeong Yoon,Wonkeun Song,Yu Bin Seo,Saeam Shin,Min Jeong Park,Seok Hoon Jeong,Kyungwon Lee +7 more
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TLDR
It is demonstrated that multidrug-resistant P. putida group carrying VIM-2 has reemerged in human specimens in Korea.Abstract:
Pseudomonas putida group are Gram-negative bacilli with polar flagellation, which are ubiquitous in the environment, although they are rarely involved in human infections. The aim of this study was to identify the dissemination of VIM-2–producing P. putida group in clinical isolates from a hospital in Korea. Thirteen strains were collected from 2014 to 2015 for the study. The isolates were recovered from urine cultures of both inpatients and outpatients at the hospital. Minimum inhibitory concentrations of antibiotics were determined by Etest. Carbapenemase genes were amplified by polymerase chain reaction and sequenced. Pulsed-field gel electrophoresis was performed for strain typing. Whole-genome sequencing was carried out randomly for two strains chosen from each year of the study to analyze the plasmid structure carrying the blaVIM-2 genes. The 13 isolates carried nine different class I integrons harboring VIM-2 and were resistant to meropenem and imipenem (minimum inhibitory concentrations, ≥32 μg/ml...read more
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Antibiotic Resistant Pseudomonas Spp. Spoilers in Fresh Dairy Products: An Underestimated Risk and the Control Strategies.
TL;DR: Light is shed on the resistome of cheese-related pseudomonads and their genomic background, current methods and advances in the prediction tools for MDR detection based on genomic sequences, possible implications for human health, and the affordable strategies to counteract MDR spread.
Journal ArticleDOI
Coordinate overexpression of two RND efflux systems, ParXY and TtgABC, is responsible for multidrug resistance in Pseudomonas putida
TL;DR: In this paper, two types of single-step multidrug-resistant (MDR) mutants were selected in vitro from reference strain KT2440, and whole-genome sequence analyses revealed that mutant HPG-5 harbours a mutation inactivating the gene (sucD) of succinyl-CoA synthetase, an enzyme of the tricarboxylic cycle.
Journal ArticleDOI
Genomic Epidemiology of MBL-Producing Pseudomonas putida Group Isolates in Poland
P Urbanowicz,Radosław Izdebski,Marta Biedrzycka,E Literacka,Waleria Hryniewicz,Marek Gniadkowski +5 more
TL;DR: In this paper , the authors performed molecular and genomic characterization of metallo-β-lactamases (MBL) producing Pseudomonas putida (MPPP) group isolates from Poland, focusing on population structures, successful genotypes and MBLencoding integrons.
Journal ArticleDOI
Molecular Characterization of Carbapenemase-Producing Gram-negative Bacteria Isolated from Clinical Specimens in Baghdad, Iraq
Saad Hamed,Noor Hasoon +1 more
TL;DR: It was concluded that the high prevalence was in blaOXA-48 gene, followed by blaVIM gene among carbapenemase-producing Gram-negative bacteria isolates, which is a worldwide emerging public health threat.
Journal ArticleDOI
Prospective evaluation of the Amplidiag® CarbaR+VRE assay for direct screening of carbapenemase producing gram-negative bacilli from rectal swabs.
Safia Nadji,Olivier Gaillot,Christophe Hallaert,Sophian Messaadi,Nourredine Loukili,Thierry Naas,Nadine Lemaitre +6 more
TL;DR: The Amplidiag® CarbaR+VRE assay could detect carbapenemases directly from rectal swabs in 3h 30 using a fully automated platform and showed high biological performances (sensitivity, specificity, and negative and positive predictive values were 100%, 98.6%, 100%, and 74.5%, respectively).
References
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Journal ArticleDOI
In Silico Detection and Typing of Plasmids using PlasmidFinder and Plasmid Multilocus Sequence Typing
Alessandra Carattoli,Ea Zankari,Aurora García-Fernández,Mette Voldby Larsen,Ole Lund,Laura Villa,Frank Møller Aarestrup,Henrik Hasman +7 more
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Metallo-β-Lactamases: the Quiet before the Storm?
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DNA sequence-based analysis of the Pseudomonas species.
TL;DR: Partial sequences of four core 'housekeeping' genes of the type strains of 107 Pseudomonas species were analysed in order to obtain a comprehensive view regarding the phylogenetic relationships within the PseUDomonas genus.
Journal ArticleDOI
Conjugative plasmids: vessels of the communal gene pool
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BookDOI
Manual of Clinical Microbiology, 11th Edition
Michael A. Pfaller,Sandra S. Richter,Guido Funke,James H. Jorgensen,Marie L. Landry,Karen C. Carroll,David W. Warnock +6 more
TL;DR: There are some minor inaccuracies in a number of the subchapters throughout the book, but the impact of these and other inaccuracies on the reading experience as a whole, is minor.