Molecular cloning and characterization of Polygalacturonase-Inhibiting Protein and Cinnamoyl-Coa Reductase genes and their association with fruit storage conditions in blueberry (Vaccinium corymbosum)
TLDR
Although the prospects of exploiting such a strategy for crop improvement are limited, the results provide further insight into the control of the quality over the storage period at the molecular level.About:
This article is published in Journal of Genetic Engineering and Biotechnology.The article was published on 2013-06-01 and is currently open access. It has received 3 citations till now.read more
Citations
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Effects of freezing conditions on quality changes in blueberries
TL;DR: Although the freezing speed for samples immersed in liquid nitrogen was faster than other treatments, samples treated at -80C showed better quality regarding vacuole water holding, drip loss, and original pectin content retention.
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Isolation, purification, and characterization of pectin methylesterase inhibitor and polygalacturonase inhibitor protein from Indian lemon (Citrus limon L.).
TL;DR: In this article, Pectin methylesterase inhibitor (PMEI) and polygalacturonase inhibitor protein (PGIP) were extracted from whole lemon fruits (Citrus limon L).
References
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Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
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Clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
TL;DR: The sensitivity of the commonly used progressive multiple sequence alignment method has been greatly improved and modifications are incorporated into a new program, CLUSTAL W, which is freely available.
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A simple and efficient method for isolating RNA from pine trees
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The leucine-rich repeat: a versatile binding motif.
Bostjan Kobe,Johann Deisenhofer +1 more
TL;DR: The crystal structure of ribonuclease inhibitor protein has revealed that leucine-rich repeats correspond to beta-alpha structural units, which are arranged so that they form a parallel beta-sheet with one surface exposed to solvent, so that the protein acquires an unusual, nonglobular shape.
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Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction.
TL;DR: A fast, simple method for constructing full-length cDNA libraries using SMART technology, which uses the template-switching activity of Moloney murine leukemia virus reverse transcriptase to synthesize and anchor first-strand cDNA in one step.